Cherenkov-excited Multi-Fluorophore Sensing in Tissue-Simulating Phantoms and In Vivo from External Beam Radiotherapy.
Cherenkov-excited Multi-Fluorophore Sensing in Tissue-Simulating Phantoms and In Vivo from External Beam Radiotherapy.
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DOI：
10.1667/rr14943.1
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发表时间：
2018-03
影响因子：
3.4
通讯作者：
Pogue BW
中科院分区：
文献类型：
--
作者：
Dsouza A;Lin H;Gunn JR;Gladstone DJ;Jarvis LA;Pogue BW
切伦科夫激发的分子传感被用来评估通过信号的光谱分离在组织模拟幻影内同时定量感测两个NIR荧光团的潜力。在500 nm至900 nm波长范围内，可检测到由外部束伽马光子辐照组织/组织模拟体模诱导的切伦科夫发射。血液的存在被证明会使检测到的切伦科夫发射的积分强度降低近50%，主要是在620 nm以下的波长处。分子染料IRDye® 680 RD和800 CW在比最强血液吸收峰更长的波长处具有激发和发射光谱，并且其中切伦科夫光的强度是最低强度，并且使得相对于背景信号的发射信号最大化。使用由1%内分泌物和1%血液组成的组织模型来模拟人乳腺组织，将含有荧光团的小瓶包埋在培养基中，并用伽马光子照射以进行切伦科夫激发。结果发现，在0.1-25 μM荧光团浓度范围内，可以在介质中5 mm深度处检测到由体模内产生的切伦科夫信号激发的荧光发射。从这些染料检测到的荧光信号与辐射剂量呈线性关系下降到cGy的水平。体内试验仅在接近1 μM的范围内成功，表明这些可用于局部浓度接近该范围的体内代谢探针。
Cherenkov-excited molecular sensing was used to assess the potential for simultaneous quantitative sensing of two NIR fluorophores within tissue simulating phantoms through spectral separation of signals. Cherenkov emissions induced by external beam gamma photon irradiation to tissues/tissue simulating phantoms were detectable over the 500 nm to 900 nm wavelength range. The presence of blood was demonstrated to reduce the integrated intensity of detected Cherenkov emissions by near 50%, predominantly at wavelengths below 620 nm. The molecular dyes IRDye® 680RD and 800CW have excitation and emission spectra at longer wavelengths than the strongest blood absorption peaks, and also where the intensity of Cherenkov light is the lowest intensity, and so that the emission signal relative to background signal is maximized. Tissue phantoms composed of 1% intralipid and 1% blood were used to simulate human breast tissue, and vials containing fluorophore were embedded in the media, and irradiated with gamma photons for Cherenkov excitation. It was found that fluorescence emissions excited by the Cherenkov signal produced within the phantom could be detected at 5 mm depth into the medium within a 0.1-25 μM fluorophore concentration range. The detected fluorescence signals from these dyes showed linear relationships with radiation doses down to the cGy level. In vivo tests were only successful within the range near a μM, suggesting that these could be used for metabolic probes in vivo where the local concentrations are near this range.