Characterization of the IgA1 protease from the Brazilian purpuric fever strain F3031 of Haemophilus influenzae biogroup aegyptius.
Characterization of the IgA1 protease from the Brazilian purpuric fever strain F3031 of Haemophilus influenzae biogroup aegyptius.
复制标题
埃及流感嗜血杆菌生物群巴西紫癜菌株 F3031 的 IgA1 蛋白酶的表征。
DOI：
10.1016/j.femsle.2005.07.010
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Actis,LuisA
中科院分区：
文献类型：
--
作者：
McGillivary,Glen;Smoot,LauraM;Actis,LuisA
Brazilian purpuric fever is a severe vascular disease caused by an invasive clone ofHaemophilus influenzaebiogroup aegyptius, which normally causes self-limiting eye infections. A previous genome subtraction procedure resulted in the isolation of a DNA fragment, which encodes a putative IgA1 protease, specific to the F3031 Brazilian purpuric fever type strain. Cloning and sequencing of the entire F3031iga1gene showed that the subtracted DNA fragment encompasses theiga1region encoding the active site and the cleavage specificity determinant of the protein, which are different from the cognate regions of the proteases produced by otherH. influenzaestrains. Western and IgA cleavage assays together with clustering analysis showed that the F3031 IgA1 protease is most similar to the type 2 proteases produced byH. influenzaetype c and e strains. Analysis of the promoter region of the F3031iga1gene revealed the presence of Fur binding sites. However, real-time PCR analysis and transcriptional fusion assays showed that the expression ofiga1is not regulated by iron or hemin under the conditions tested.
