Effects of toxic dinoflagellate Alexandrium tamarense on the energy budgets and growth of two marine bivalves
Effects of toxic dinoflagellate Alexandrium tamarense on the energy budgets and growth of two marine bivalves
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DOI：
10.1016/s0141-1136(01)00117-9
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发表时间：
2002-03-01
影响因子：
3.3
通讯作者：
Hsieh, DPH
中科院分区：
文献类型：
--
作者：
Li, SC;Wang, WX;Hsieh, DPH
有害藻华(HAB)对水生生物和人类健康构成严重威胁。我们测定了有毒甲藻Alexandrium Tamarense(ATC101克隆，从香港沿海水域分离)对能量收支的影响，量化为生长范围(SFG)，并测定了菲律宾蛤仔和翡翠贻贝的生长速度。为了定量测定SFG，在文蛤和贻贝中加入不同剂量的有毒塔马氏菌，连续6天，导致组织中不同的毒素负荷。随后测量了这些含毒素双壳类的排泄率、吸收效率和呼吸速率。毒素负荷量最高时，贻贝的排泄率显著下降，而不同处理间的排泄率差异不显著。两种双壳贝类的呼吸速率都不受组织中毒素积累的显著影响。相反，当组织积累毒素时，贻贝和贻贝的吸收效率都会下降，这对贻贝来说是一种浓度依赖的方式，但对贻贝来说并非如此。随着麻痹性贝毒(PSP)毒素负荷的增加，文蛤和贻贝体内的SFG显著降低，这主要是由于吸收效率的降低。在摄食有毒甲藻的过程中，菲律宾贝幼虾的生长速度显著低于以硅藻为食的文蛤的生长速度。然而，翡翠贻贝幼体在摄食有毒的甲藻和无毒的硅藻后，表现出相似的生长速度。本研究表明，SFG能灵敏而全面地反映赤潮对双壳类生物生理的影响。菲律宾蛤仔在能量收支方面可能比翡翠贻贝对PSP毒素的积累更为敏感。(C)2001年，爱思唯尔科学有限公司出版。版权所有。
Harmful algal blooms (HAB) may impose a serious threat to aquatic lives and human health. We determined the effects of a toxic dinoflagellate Alexandrium tamarense (clone ATC101, isolated from Hong Kong coastal waters) on the energy budget, quantified as scope for growth (SFG), and the growth rate of the manila clam Ruditapes philippinarum and the green-lipped mussel Perna viridis. To quantify the SFG, clams and mussels were dosed with different amounts of toxic A. tamarense for 6 days, resulting in different toxin burdens in the tissues. Clearance rate, absorption efficiency, and respiration rate were subsequently measured in these toxin containing bivalves. Clearance rate significantly declined at the highest toxin burden in the clams only, while there was no significant difference in the clearance rate among different treatments for the mussels. The respiration rate of either bivalve was not significantly affected by toxin accumulation in the tissues. In contrast, the absorption efficiency of both clams and mussels decreased, in a concentration-dependent manner for mussels but not for clams, when the tissue accumulated the toxins. With an increase in paralytic shellfish poisoning (PSP) toxin burden, SFG in both clams and mussels was significantly reduced, primarily because of the decrease of absorption efficiency. The growth rate of juvenile clam R. philippinarum, measured as an increment in tissue dry weight over a 15 d exposure period, was significantly lower during their feeding on toxic dinoflagellate than the growth rate of clams feeding on the diatom Thalassiosira pseudonana. The juvenile mussel P. viridis, however, exhibited similar growth rates after feeding on the toxic dinoflagellates and the nontoxic diatom. This study showed that SFG can provide a sensitive and integrated measure of the effect of HAB on the physiology of bivalves. Clam R. philippinarum may be more sensitive, in terms of their energy budget, to PSP toxin accumulation than the mussel P. viridis. (C) 2001 Published by Elsevier Science Ltd. All rights reserved.