Aberrant expression of liver microRNA in chickens infected with subgroup J avian leukosis virus
Aberrant expression of liver microRNA in chickens infected with subgroup J avian leukosis virus
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J亚型禽白血病病毒感染鸡肝脏微小RNA的异常表达
DOI：
10.1016/j.virusres.2012.07.003
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发表时间：
2012-10-01
期刊：
影响因子：
5
通讯作者：
Bi, Yingzuo
中科院分区：
文献类型：
--
作者：
Li, Hongmei;Ji, Jun;Bi, Yingzuo
J亚型禽白血病病毒（ALV-J）是一种主要引起肉鸡髓系白血病（ML）的致癌逆转录病毒。尽管ALV在包括美国在内的少数国家得到了很好的控制，但由于散发或广泛的ALV感染，特别是ALV- j感染，世界许多地区的家禽业继续遭受严重的经济损失。据报道，鸡的ALV-J感染是由细胞受体介导的。然而，到目前为止，还没有发现对ALV-J产生抗性的受体基因的遗传变异。为了进一步了解参与ALV-J感染事件的表观遗传因素，我们通过miRNA芯片分析检测了未感染或感染ALV-J的10周龄鸡肝脏中miRNA的表达。我们的数据显示，在未感染组和感染组的肝脏中有12个mirna的差异表达（P < 0.01）。其中，7种mirna （gga-mir-221、gga-mir-222、gga-mir-1456、gga-mir-1704、gga-mir-1777、gga-mir-1790和gga-mir-2127）的表达因ALV-J感染而上调，可能与致癌性有关。其他5种mirna （gga-let-7b、gga-let-7i、gga-mir-125b、gga-mir-375和gga-mir-458）显著下调。下调的mirna可能在肿瘤抑制中发挥重要作用。这一发现为进一步探索表观遗传对ALV-J感染致瘤性的影响铺平了道路。(C) 2012 Elsevier B.V.版权所有
Subgroup J avian leukosis virus (ALV-J) is an oncogenic retrovirus primarily causing myeloid leukosis (ML) in broilers. Although ALV is well under control in a few countries including the USA, poultry industry in many parts of the world continues suffering from serious economic loss due to sporadic or widespread ALV infection, especially ALV-J infection. ALV-J infection of chickens is reportedly mediated by a cellular receptor. So far, however, no genetic variant of the receptor gene that confers resistance to ALV-J has been identified. To advance our understanding on epigenetic factors that are involved in the event of ALV-J infection, we examined the expression of miRNAs in livers of 10-week-old chickens uninfected or infected with ALV-J by miRNA microarray analysis. Our data showed there were 12 miRNAs differentially expressed in liver between the uninfected and infected groups (P < 0.01). Of which, the expressions of seven miRNAs (gga-mir-221, gga-mir-222, gga-mir-1456, gga-mir-1704, gga-mir-1777, gga-mir-1790, and gga-mir-2127,) were upregulated by ALV-J infection and may be involved in oncogenicity. The other five miRNAs (gga-let-7b, gga-let-7i, gga-mir-125b, gga-mir-375, and gga-mir-458) were significantly downregulated. The downregulated miRNAs may play important roles in tumor suppression. This finding paves the way for further exploration of epigenetic influence on tumorigenicity upon ALV-J infection. (C) 2012 Elsevier B.V. All rights reserved.