Antibacterial activity and biofilm inhibition by surface modified titanium alloy medical implants following application of silver, titanium dioxide and hydroxyapatite nanocoatings
Antibacterial activity and biofilm inhibition by surface modified titanium alloy medical implants following application of silver, titanium dioxide and hydroxyapatite nanocoatings
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DOI：
10.1080/17435390.2017.1299890
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发表时间：
2017-04-01
期刊：
影响因子：
5
通讯作者：
Handy, R. D.
中科院分区：
文献类型：
--
作者：
Besinis, A.;Hadi, S. D.;Handy, R. D.
种植体失败的最常见原因之一是种植体周围炎，这是由牙种植体表面上的细菌生物膜形成引起的。表面纳米形貌的修饰已被认为会影响细菌对植入物的粘附。银纳米颗粒也因其抗菌性能而闻名。在这项研究中，钛合金植入物表面改性后，银电镀，阳极氧化和烧结技术，以创建一个组合的银，二氧化钛和羟基磷灰石（HA）纳米涂层。通过测定24 h内血链球菌的生长、活/死细胞比例和乳酸产生量来定量评价其抗菌性能。将双层银-HA纳米涂层应用于植入物表面成功地抑制了周围介质中的细菌生长（100%死亡率），而植入物表面上细菌生物膜的形成减少了97.5%。未涂覆的对照和二氧化钛纳米涂层显示没有抗菌效果。发现银和HA纳米涂层在生物流体中非常稳定，由于溶解，银纳米涂层在改良的Krebs-Ringer碳酸氢盐缓冲液中24小时后的材料损失小于0.07%。未检测到HA纳米涂层的溶解。因此，将双层银-HA纳米涂层应用于钛合金植入物可产生具有抗氧化膜性质的表面，而不会损害成功骨整合和加速骨愈合所需的HA生物相容性。
One of the most common causes of implant failure is peri-implantitis, which is caused by bacterial biofilm formation on the surfaces of dental implants. Modification of the surface nanotopography has been suggested to affect bacterial adherence to implants. Silver nanoparticles are also known for their antibacterial properties. In this study, titanium alloy implants were surface modified following silver plating, anodisation and sintering techniques to create a combination of silver, titanium dioxide and hydroxyapatite (HA) nanocoatings. Their antibacterial performance was quantitatively assessed by measuring the growth of Streptococcus sanguinis, proportion of live/dead cells and lactate production by the microbes over 24h. Application of a dual layered silver-HA nanocoating to the surface of implants successfully inhibited bacterial growth in the surrounding media (100% mortality), whereas the formation of bacterial biofilm on the implant surfaces was reduced by 97.5%. Uncoated controls and titanium dioxide nanocoatings showed no antibacterial effect. Both silver and HA nanocoatings were found to be very stable in biological fluids with material loss, as a result of dissolution, to be less than 0.07% for the silver nanocoatings after 24h in a modified Krebs-Ringer bicarbonate buffer. No dissolution was detected for the HA nanocoatings. Thus, application of a dual layered silver-HA nanocoating to titanium alloy implants creates a surface with antibiofilm properties without compromising the HA biocompatibility required for successful osseointegration and accelerated bone healing.