Cloning and characterization of a Leishmania gene encoding a RNA spliced leader sequence.
Cloning and characterization of a Leishmania gene encoding a RNA spliced leader sequence.
复制标题
编码 RNA 剪接前导序列的利什曼原虫基因的克隆和表征。
DOI：
10.1093/nar/14.18.7341
复制
发表时间：
1986
影响因子：
14.9
通讯作者：
Wirth,DF
中科院分区：
文献类型：
--
作者：
Miller,SI;Landfear,SM;Wirth,DF
最近对利什曼原虫微管蛋白基因的研究发现其5′端增加了35个核苷酸。编码这个35个核苷酸前导序列的基因现在已经被克隆和测序。在利什曼原虫基因组中，剪接的前导基因以438个碱基的串联重复形式存在。该基因约有150个拷贝，占寄生虫基因组的0.1%。该基因编码一个85个核苷酸的转录本，在其5′端含有剪接的前导序列。该35个核苷酸序列及其5′和3′端区域在锥虫中高度保守。我们已经检测到一种RNA分子，它是加工反应的假定副产物，在该反应中，35个核苷酸的剪接前导序列已转移到mRNA。我们认为，这个分子是残余的剪接前导转录后删除的35个核苷酸剪接的领导人。
Recent studies onLeishmaniaenriettiitubulin mRNAs revealed a 35 nucleotide addition to their 5′ end. The gene that codes for this 35 nucleotide leader sequence has now been cloned and sequenced. In theLeishmaniagenome, the spliced leader gene exists as a tandem repeat of 438 bases. There are approximately 150 copies of this gene comprising 0.1% of the parasite genome. This gene codes for a 85 nucleotide transcript that contains the spliced leader at its 5′ end. The 35 nucleotide sequence and the regions immediately 5′ and 3′ to it are highly conserved across trypanosomatids. We have detected a RNA molecule that is a putative by-product of the processing reaction in which the 35 nucleotide spliced leader has been transferred to mRNA. We suggest that this molecule is the remnant of the spliced leader transcript after removal of the 35 nucleotide spliced leader.