Intramuscular inoculation of Sin Nombre hantavirus cDNAs induces cellular and humoral immune responses in BALB c mice
Intramuscular inoculation of Sin Nombre hantavirus cDNAs induces cellular and humoral immune responses in BALB c mice
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DOI：
10.1016/s0264-410x(99)00096-1
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发表时间：
1999-07-16
期刊：
影响因子：
5.5
通讯作者：
Hjelle, B
中科院分区：
文献类型：
--
作者：
Bharadwaj, M;Lyons, CR;Hjelle, B
为了研究用Sin Nombre：（SN）汉坦病毒基因进行基因免疫是否能引起免疫应答，将跨越包膜糖蛋白基因G1和G2的9个片段以及完整的N基因克隆到CMV表达载体中。为了确保所有潜在表位的代表性，糖蛋白基因的相邻片段彼此重叠100个核苷酸。将含有基因片段的载体肌肉内接种到BALB/c小鼠中，并测定脾细胞增殖和Western印迹可检测的抗体和中和滴度。N基因和7个的9个M段衍生的cDNA测试产生显着的特异性淋巴增生反应，许多结构引起中和或western印迹可检测的抗体。这些有希望的结果鼓励SN病毒的感染模型的发展，将能够检测保护性反应。(C)1999 Elsevier Science Ltd.保留所有权利。
To examine whether genetic immunization with Sin Nombre: (SN) hantavirus genes could elicit immune responses, nine fragments spanning the envelope glycoprotein genes G1 and G2, and the complete N gene were cloned into a CMV expression vector. To ensure representation of all potential epitopes, adjacent fragments of the glycoprotein genes overlapped one another by 100 nucleotides. Vectors containing the gene fragments were inoculated intramuscularly into BALB/c mice and splenocyte proliferation and western blot-detectable antibodies and neutralization titers were determined. The N gene and seven of the nine M segment-derived cDNAs tested produced significant specific lymphoproliferative responses, and many of the constructs elicited either neutralizing or western blot-delectable antibodies. These promising results encourage the development of infection models for SN virus that will be capable of detecting protective responses. (C) 1999 Elsevier Science Ltd. All rights reserved.