Antidepressant-like Effect of Fuzi Total Alkaloid on Ovariectomized Mice
Antidepressant-like Effect of Fuzi Total Alkaloid on Ovariectomized Mice
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附子总生物碱对去卵巢小鼠的抗抑郁样作用
DOI：
10.1254/jphs.12163fp
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发表时间：
2012-12-01
影响因子：
3.5
通讯作者：
Li, Yuxin
中科院分区：
文献类型：
--
作者：
Liu, Lei;Li, Bingjin;Li, Yuxin
近年来的体内外研究表明，附子多糖具有抗抑郁作用。附子中最重要的两个成分是多糖和总生物碱。然而，关于附子总生物碱的抗抑郁作用却知之甚少。为探讨附子总生物碱的抗抑郁作用，采用旷场实验和强迫游泳实验对其进行了行为学研究。给正常小鼠连续灌胃附子总生物碱7d(10 mg/kg)，可明显缩短小鼠的不动时间。此外，给去卵巢小鼠重复给予附子总生物碱(10 mg/kg或30 mg/kg)也可剂量依赖性地减少小鼠的不动时间。然而，这些抗抑郁药样的行为效应并不简单地归因于运动性多动。进一步实验表明，附子总生物碱能提高去卵巢小鼠额叶皮质和海马区磷酸化CREB/CREB(cAMP反应元件结合)和BDNF(脑源性神经营养因子)蛋白水平的比值，但对正常小鼠无明显影响。上述结果提示，CREB-BDNF通路可能参与了附子总生物碱对去卵巢小鼠的抗抑郁作用。[补充数字：仅在http://dx.doi.org/10.1254/jphs.12163FP]上提供
Recent studies in vivo and vitro have shown that Fuzi polysaccharide has an antidepressant-like effect. Polysaccharide and total alkaloid are the two most important components of Fuzi. However, little is known about the antidepressant-like effect of Fuzi total alkaloid. To investigate the antidepressant-like effect of Fuzi total alkaloid, behavioral studies were performed in the open field test and forced swimming test. Repeated intragastric administration of Fuzi total alkaloid for 7 days (10 mg/kg) to normal mice decreased immobility time compared to the vehicle group. Furthermore, repeated administration of Fuzi total alkaloid (10 or 30 mg/kg) to ovariectomized mice also decreased immobility time in a dose-dependent manner. However, these antidepressant-like behavioral effects were not simply due to locomotor hyperactivity. Further experiments showed that Fuzi total alkaloid enhanced the ratio of phospho-CREB/CREB (cAMP response element-binding) and BDNF (brain-derived neurotrophic factor) protein level in the frontal cortex and hippocampus in ovariectomized mice but not in normal mice. These results indicate that the CREB-BDNF pathway may be involved in the antidepressant-like effect of Fuzi total alkaloid in ovariectomized mice. [Supplementary Figures: available only at http://dx.doi.org/10.1254/jphs.12163FP]