The yeast Mcm1 protein is regulated posttranscriptionally by the flux of glycolysis.
The yeast Mcm1 protein is regulated posttranscriptionally by the flux of glycolysis.
复制标题
酵母 Mcm1 蛋白受糖酵解通量的转录后调节。
DOI：
10.1128/mcb.15.8.4631
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发表时间：
1995
影响因子：
5.3
通讯作者：
Tye,BK
中科院分区：
文献类型：
--
作者：
Chen,Y;Tye,BK
Mcm1 是一种多功能蛋白，在酿酒酵母 DNA 复制的启动和多种基因的转录调控中发挥作用。 Themcm1-1突变导致微型染色体不稳定和α特异性不育。分离出能够恢复微染色体稳定性但不恢复 cm1-1 突变体生育力的第二位点抑制子。其中两个抑制因子 pgm1-1 和 pgm1-2 是 PGM1 的突变等位基因，编码糖酵解酶、磷酸甘油酸变位酶。我们表明，pgm1-1 突变通过增加转录后 Mcm1-1 的蛋白质活性或水平来抑制微型染色体维持 (Mcm) 缺陷。细胞内 Mcm1-1 活性的增加足以抑制 Mcm 缺陷，但仅最小程度地抑制交配缺陷。编码其他糖酵解酶（例如aseno2::URA3）的基因突变也可以抑制mcm1-1的Mcm表型。这些糖酵解酶突变的抑制与糖酵解速率降低相关，而不是与细胞生长速率降低相关。这项研究表明，为了应对营养状态的变化，酵母细胞可能通过调节 Mcm1 等全局调节因子的活性来实现体内平衡，而 Mcm1 在调节耗能的合成代谢过程中发挥着核心作用。
Mcm1 is a multifunctional protein which plays a role both in the initiation of DNA replication and in the transcriptional regulation of diverse genes inSaccharomyces cerevisiae. Themcm1-1mutation results in instability of minichromosomes and α-specific sterility. Second-site suppressors that restore minichromosome stability but not fertility to themcm1-1mutant were isolated. Two of the suppressors,pgm1-1andpgm1-2, are mutant alleles ofPGM1which encodes a glycolytic enzyme, phosphoglycerate mutase. We show that thepgm1-1mutation suppresses the minichromosome maintenance (Mcm) defect by increasing the protein activity or level of Mcm1-1 posttranscriptionally. This increase in the intracellular Mcm1-1 activity is sufficient to suppress the Mcm defect but only minimally suppresses the mating defect. Mutations in genes encoding other glycolytic enzymes, such aseno2::URA3, can also suppress the Mcm phenotype ofmcm1-1. Suppression by these glycolytic enzyme mutations correlates with a reduced rate of glycolysis rather than a reduced rate of cell growth. This study suggests that in response to changes in their nutritional states yeast cells may attain homeostasis by modulating the activity of global regulators like Mcm1, which plays a central role in the regulation of energy-expensive anabolic processes.