Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging
Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging
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用于扩大视野、多平面和长期脑成像的微型双光子显微镜
DOI：
10.1038/s41592-020-01024-z
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发表时间：
2021-01-01
期刊：
影响因子：
48
通讯作者：
Cheng, Heping
中科院分区：
文献类型：
--
作者：
Zong, Weijian;Wu, Runlong;Cheng, Heping
我们已经开发了一种微型双光子显微镜，配备了轴向扫描机制和长工作距离的微型物镜，使多平面成像超过420x420x180 μ m（3）的体积在横向分辨率类似于1 μ m。结合允许长期重复成像的可拆卸设计，我们的微型双光子显微镜可以帮助破译自由行为动物的神经机制。
We have developed a miniature two-photon microscope equipped with an axial scanning mechanism and a long-working-distance miniature objective to enable multi-plane imaging over a volume of 420x420x180 mu m(3) at a lateral resolution of similar to 1 mu m. Together with the detachable design that permits long-term recurring imaging, our miniature two-photon microscope can help decipher neuronal mechanisms in freely behaving animals.