Developmental changes in the expression of the GLUT2 and GLUT4 genes in the longissimus dorsi muscle of Yorkshire and Tibetan pigs
Developmental changes in the expression of the GLUT2 and GLUT4 genes in the longissimus dorsi muscle of Yorkshire and Tibetan pigs
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DOI：
10.4238/2015.february.13.7
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发表时间：
2015-01-01
影响因子：
0.4
通讯作者：
Lv, X. B.
中科院分区：
文献类型：
--
作者：
Liang, Y.;Yang, X. M.;Lv, X. B.
葡萄糖转运蛋白2和4（GLUT 2和GLUT 4）在葡萄糖转运和能量代谢中发挥重要作用。采用实时荧光定量聚合酶链反应（RT-PCR）技术，测定了1、2、3、4、5、6月龄（6个时间点）瘦肉型约克郡猪和肥胖型藏猪背最长肌GLUT 2和GLUT 4 mRNA水平的变化。结果表明，GLUT 2和GLUT 4 mRNA在猪背最长肌中大量表达，且两品种猪背最长肌中GLUT 2和GLUT 4 mRNA的发育表达模式相似。藏猪肌内脂肪含量和GLUT 2 mRNA水平较高，而约克郡猪肌纤维横截面积（CSA）和GLUT 4 mRNA水平较高。此外，GLUT 4 mRNA水平的变化与CSA在6个月内呈强正相关。这些结果显示了GLUT 2和GLUT 4的时间和品种特异性表达模式，这突出了它们作为评估猪脂肪沉积和肌肉发育的候选基因的潜力。GLUT家族基因表达的这些差异也可能对肉质有指示作用。
Glucose transporter proteins 2 and 4 (GLUT2 and GLUT4) play important roles in glucose transport and energy metabolism. Changes in the levels of GLUT2 and GLUT4 mRNA were measured in longissimus dorsi muscle from the lean Yorkshire and fat Tibetan pig breeds at six different time points (1, 2, 3, 4, 5, and 6 months) with quantitative real-time polymerase chain reactions. The results showed that GLUT2 and GLUT4 mRNA were abundantly expressed in the longissimus dorsi muscle and that the developmental expression patterns were similar in both breeds. Tibetan pigs exhibited higher intramuscular fat and GLUT2 mRNA levels, while Yorkshire pigs exhibited a higher myofiber cross-sectional area (CSA) and GLUT4 mRNA levels. Furthermore, the changes in the GLUT4 mRNA levels were strongly and positively correlated with the CSA over a period of six months. These results exhibit time- and breed-specific expression patterns of GLUT2 and GLUT4, which highlight their potential as candidate genes for assessing adipose deposition and muscle development in pigs. These differences in the expression of GLUT family genes may also have indications for meat quality.