Melanoma cell adhesion to basement membrane mediated by integrin-related complexes.
Melanoma cell adhesion to basement membrane mediated by integrin-related complexes.
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DOI：
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发表时间：
1989-01
期刊：
影响因子：
11.2
通讯作者：
R. Kramer;K. Mcdonald;E. Crowley;D. M. Ramos;C. Damsky
中科院分区：
文献类型：
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作者：
R. Kramer;K. Mcdonald;E. Crowley;D. M. Ramos;C. Damsky
在侵袭和转移过程中，肿瘤细胞利用多种表面粘附受体附着并侵入基底膜和间质。我们研究了细胞表面整合素样复合物在侵袭性小鼠 B16-BL6 黑色素瘤细胞系附着至基底膜中的作用。针对从仓鼠 BHK 细胞（抗 ECMR）或小鼠红白血病细胞（抗小鼠 FnR）分离的整合素相关复合物制备的多克隆抗体抑制 B16 细胞附着于复杂的基底膜基质和涂有纯化的细胞外基质成分（纤连蛋白、层粘连蛋白和 IV 型胶原）的基质上。通过用抗体对放射性标记和溶解的膜蛋白进行选择性免疫沉淀，证实了 B16 细胞表面整合素样受体的表达。两种抗体还与整合素相关的纤连蛋白结合受体复合物发生反应，该复合物通过纤连蛋白-琼脂糖柱上的配体亲和层析纯化。抗整联蛋白抗体未能与 Mr 68,000 层粘连蛋白结合蛋白反应，表明它们对细胞附着于层粘连蛋白和复杂基底膜的抑制并不是由于针对 Mr 68,000 层粘连蛋白受体的污染抗体。结果表明，B16-BL6 细胞上的整合素受体复合物要么直接与多种细胞外基质相关成分相互作用，要么以某种方式调节其他受体的活性和功能。因此，整合素可能在肿瘤细胞侵袭组织屏障中发挥重要作用。
During invasion and metastasis, tumor cells use a variety of surface adhesion receptors to attach to and invade basement membranes and interstitial stroma. We examined the role of the cell surface integrin-like complex in the attachment of the invasive murine B16-BL6 melanoma cell line to basement membrane. Polyclonal antibodies prepared against integrin-related complexes isolated from hamster BHK cells (anti-ECMR) or mouse erythroleukemia cells (anti-mouse FnR) inhibited the attachment of B16 cells to complex basement membrane matrices and to substrates coated with purified extracellular matrix components (fibronectin, laminin, and type IV collagen). The expression of integrin-like receptors on the surface of B16 cells was confirmed by selective immunoprecipitation of radiolabeled and solubilized membrane proteins with the antibodies. Both antibodies also reacted with an integrin-related fibronectin-binding receptor complex purified by ligand affinity chromatography on fibronectin-Sepharose columns. The anti-integrin antibodies failed to react with the Mr 68,000 laminin-binding protein, suggesting that their inhibition of cell attachment to laminin and complex basement membrane was not due to contaminating antibodies against the Mr 68,000 laminin receptor. The results indicate that the integrin receptor complexes on B16-BL6 cells either interact directly with a diverse set of extracellular-matrix-associated components or somehow modulate the activity and function of other receptors. Thus integrins may have an important role in tumor cell invasion of tissue barriers.