Zebrafish Danio rerio myotomal muscle structure and growth from a spatial transcriptomics perspective
Zebrafish Danio rerio myotomal muscle structure and growth from a spatial transcriptomics perspective
复制标题
从空间转录组学角度观察斑马鱼斑马鱼肌节肌肉结构和生长
DOI：
10.1016/j.ygeno.2022.110477
复制
发表时间：
2022
期刊：
影响因子：
4.4
通讯作者：
Shigeharu Kinoshita
中科院分区：
文献类型：
--
作者：
Guanting Liu;Takumi Ito;Yusuke Kijima;Kazutoshi Yoshitake;Shuichi Asakawa;Shugo Watabe;Shigeharu Kinoshita
与哺乳动物相比，鱼类具有不同的肌肉结构和生长特征。我们使用了空间转录组学的方法，并检查了从斑马鱼肌节肌肉切片。根据空间基因表达特征将成年肌肉分为8个区域。慢肌位于背鳍基部靠近侧线的楔形区域，中间肌位于与慢肌相邻的带状区域，快肌位于躯干深部，被中间肌包围;快肌内部根据转录组学特征进一步分为6个部分。成人和幼虫数据的综合分析表明，成人肌肉包含特定的区域类似于幼虫的肌肉。这些区域显示出活跃的肌生成和与肌肉增生相关的基因的高表达。这是首次将空间转录组学应用于鱼类肌节肌肉结构和生长的研究。
Fish exhibit different muscle structures and growth characteristics compared with mammals. We used a spatial transcriptomics approach and examined myotomal muscle sections from zebrafish. Adult muscles were divided into eight regions according to spatial gene expression characteristics. Slow muscle was located in the wedge-shaped region near the lateral line and at the base of the dorsal fin, intermediate muscle was located in a ribbon-shaped region adjacent to slow muscle, and fast muscle was located in the deep region of the trunk, surrounded by intermediate muscle; the interior of fast muscle was further divided into 6 parts by their transcriptomic features. Combined analysis of adult and larval data revealed that adult muscles contain specific regions similar to larval muscles. These regions showed active myogenesis and a high expression of genes associated with muscle hyperplasia. This is the first study to apply spatial transcriptomics to fish myotomal muscle structure and growth.