Rapid quantitation of curcumin in turmeric via NMR and LC-tandem mass spectrometry
Rapid quantitation of curcumin in turmeric via NMR and LC-tandem mass spectrometry
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DOI：
10.1016/j.foodchem.2008.08.014
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发表时间：
2009-04-15
期刊：
影响因子：
8.8
通讯作者：
Bilsel, Gokhan
中科院分区：
文献类型：
--
作者：
Goren, Ahmet C.;Cikrikci, Simay;Bilsel, Gokhan
我们开发了一种快速、灵敏且准确的 (1) H NMR 方法，用于定量从姜黄根茎（姜黄）提取物中分离的姜黄素，并将其结果与经过验证的 LC-MS/MS 方法进行比较。 (1)H NMR 和 LC-MS/MS 方法的相对标准偏差分别为 2.49% 和 3.48%。在校准范围内，(1)H NMR 的相关系数为 0.998，LC-MS/MS 测定的相关系数为 0.995。两种技术在 5 mg mL(-1) 和 50 mu g mL(-1) 浓度下的平均回收率分别为 99-101%。 (1)H NMR 光谱测定姜黄素的不确定度为 5.80%，而 LC-ESI-MS/MS 方法的不确定度为 7.38%。 (C) 2008 Elsevier Ltd. 保留所有权利。
A rapid, sensitive and accurate (1)H NMR method has been developed for the quantitation of curcumin isolated from Curcuma longo rhizome (turmeric) extract, the results of which were compared with a validated LC-MS/MS method. The relative standard deviations of the methods were found to be 2.49% and 3.48% for the (1)H NMR and LC-MS/MS methods, respectively. The correlation coefficients were 0.998 for (1)H NMR and 0.995 for LC-MS/MS assay in the calibration range. The recoveries at 5 mg mL(-1) and 50 mu g mL(-1) concentrations averaged to 99-101% for both techniques, respectively. The uncertainty of the measurement Of curcumin via (1)H NMR spectroscopy was determined to he 5.80% while in LC-ESI-MS/MS method was 7.38%. (C) 2008 Elsevier Ltd. All rights reserved.