Simultaneous determination of aflatoxins and ochratoxin A in baby foods and paprika by HPLC with fluorescence detection: A single-laboratory validation study
Simultaneous determination of aflatoxins and ochratoxin A in baby foods and paprika by HPLC with fluorescence detection: A single-laboratory validation study
复制标题
DOI：
10.1016/j.talanta.2010.11.031
复制
发表时间：
2011-02-15
期刊：
影响因子：
6.1
通讯作者：
Miraglia, M.
中科院分区：
文献类型：
--
作者：
Brera, C.;Debegnach, F.;Miraglia, M.
真菌毒素是真菌来源的毒性次生代谢产物，食品关注的主要真菌毒素是黄曲霉毒素和赭曲霉毒素A.由于真菌毒素污染的基质范围广，可能共存，浓度范围广，因此迫切需要验证多功能的多真菌毒素和多基质方法。建立了同时测定婴儿食品和辣椒粉中黄曲霉毒素和赭曲霉毒素A含量的反相高效液相色谱法。根据商业可用性准备了三种散装样品，一种用于辣椒粉，一种用于婴儿食品，两种不同的散装，一种以玉米为基础，一种以多种谷物为基础的婴儿食品。进行单实验室验证，对每个被调查的水平进行10次分析，计算重复性的相对标准偏差（RSDr）和恢复因子；AFB(1)的RSDr值为2% ~ 10%，OTA的RSDr值为3% ~ 10%，而AFB(1)的回收率为86% ~ 96%，OTA的回收率为77% ~ 96%。检查了RSDr和回收率与当前欧盟法规中报告的值的合规性，确认了该方法的适用性。该方法对婴儿食品中AFB(1)的检出限分别为0.002和0.020 μ g/kg，对OTA的检出限分别为0.012和0.080 μ g/kg；红辣椒中AFB(1)含量分别为0.002和0.200 μ g/kg， OTA含量分别为0.012和0.660 μ g/kg。目前的方法是根据实验室研究或官方控制目的进行多种霉菌毒素和/或多种基质分析的可能性的一个很好的例子。(C) 2010 Elsevier B.V.版权所有
Mycotoxins are toxic secondary metabolites of fungal origin, the major mycotoxins of food concern are aflatoxins and ochratoxin A. Due to the wide range of matrices susceptible to mycotoxin contamination, the possible co-occurrence, and the very wide range of concentration, validated versatile multi-mycotoxin and multi-matrix methods are strongly requested. A reversed phase HPLC method for the simultaneous determination of aflatoxins and ochratoxin A in baby foods and paprika was set up. Three bulk samples were prepared according to commercial availability, one for paprika and for baby foods, two different bulks were set, a corn based and a multi-cereal based baby food. A single-laboratory validation was performed, for each investigated level ten analyses were performed, relative standard deviations of repeatability (RSDr) and recovery factors were calculated; RSDr values ranged from 2% to 10% for AFB(1) and from 3% to 10% for OTA, while the recovery factors ranged from 86% to 96% for AFB(1) and from 77% to 96% for OTA. The checked compliance of the RSDr and recovery with the values reported in the current EU Regulations confirmed the fitting for purpose of the method. Limit of detection and LoQ values of the method were respectively 0.002 and 0.020 mu g/kg for AFB(1) and 0.012 and 0.080 mu/kg for OTA in baby foods; and 0.002 and 0.200 mu g/kg for AFB(1) and 0.012 and 0.660 mu g/kg for OTA in paprika. The current method represents a good example of the possibility of a multi-mycotoxin and/or a multi-matrix analysis depending on the laboratory research or official control purposes. (C) 2010 Elsevier B.V. All rights reserved.