Loss of gdnfa disrupts spermiogenesis and male courtship behavior in zebrafish
Loss of gdnfa disrupts spermiogenesis and male courtship behavior in zebrafish
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DOI：
10.1016/j.mce.2023.112010
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发表时间：
2023-07
影响因子：
4.1
通讯作者：
Xianyao Liao;Binbin Tao;Xiya Zhang;Lu Chen;Ji Chen;Yanlong Song;Weizhong Hu
中科院分区：
文献类型：
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作者：
Xianyao Liao;Binbin Tao;Xiya Zhang;Lu Chen;Ji Chen;Yanlong Song;Weizhong Hu
精子发生对雄性脊椎动物生殖系统的建立和维持至关重要。精子发生是高度保守的，主要受激素、生长因子和表观遗传因子的共同调控。胶质细胞源性神经营养因子（Glial cell line-derived neurotrophic factor，GDNF）是转化生长因子-β超家族的成员。本研究建立了gdnfa基因敲除和Tg（gdnfa：mcherry）转基因斑马鱼品系。gdn的丢失导致睾丸组织紊乱，性腺指数下降，成熟精子百分比降低。在Tg（gdnfa：mcherry）斑马鱼品系中，我们发现gdnfa在Leydig细胞中表达。该突变显著降低了Leydig细胞标记基因的表达和Leydig细胞雄激素的分泌。此外，雄性突变体的求偶行为也受到了干扰。我们在活体研究中发现GDNF基因的敲除会影响斑马鱼的精子发生和雄性求偶行为，这是第一个具有GDNF基因敲除的脊椎动物模型，对于研究GDNF在动物生殖中的作用具有重要意义。
Spermatogenesis is essential for establishment and maintenance of reproduction in male vertebrates. Spermatogenesis, which is mainly regulated by the combined action of hormones, growth factors, and epigenetic factors, is highly conserved. Glial cell line-derived neurotrophic factor (GDNF) is a member of the transforming growth factor-β superfamily. In this study, globalgdnfaknockout andTg (gdnfa: mcherry)transgenic zebrafish lines were generated. Loss ofgdnfaresulted in disorganized testes, decreased gonadosomatic index, and low percentage of mature spermatozoa. In theTg (gdnfa: mcherry)zebrafish line, we found thatgdnfawas expressed in Leydig cells. The mutation ingdnfasignificantly decreased Leydig cell marker gene expression and androgen secretion in Leydig cells. In addition, courtship behavior was disrupted in the male mutants. We present in vivo data showing that global knockout ofgdnfadisrupts spermiogenesis and male courtship behavior in zebrafish.The first viable vertebrate model with a globalgdnfaknockout may be valuable for studying the role of GDNF in animal reproduction.