7-Amino-4-methylcoumarin as a fluorescent substitute for Schiff’s reagent: a new method that can be combined with hemalum and eosin staining on the same tissue section
7-Amino-4-methylcoumarin as a fluorescent substitute for Schiff’s reagent: a new method that can be combined with hemalum and eosin staining on the same tissue section
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7-氨基-4-甲基香豆素作为希夫试剂的荧光替代品：一种可以在同一组织切片上结合血红和伊红染色的新方法
DOI：
10.1080/10520295.2022.2101144
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Inagaki Hiroshi
中科院分区：
文献类型：
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作者：
Takase Hiroshi;Murase Takayuki;Hachisuka Daisuke;Sakamoto Yuma;Sugiura Mariko;Nakano Satsuki;Fujii Keiichiro;Masaki Ayako;Inagaki Hiroshi
7-氨基-4-甲基香豆素（AMC）水溶液在紫外光（UV）下显示出强荧光，可用作希夫试剂来显示醛。我们研究了血红素和伊红（H & E）和AMC染色的组织学和病理学分析。用组合的H & E/AMC染色对正常和病变的人组织的切片进行染色。H & E/AMC染色后，在明视野显微镜下保留H & E形态。在紫外光下观察到的AMC荧光信号强烈，染色模式与通过过碘酸-希夫（PAS）染色获得的染色模式相同。存档的H & E切片的AMC染色也是成功的。淀粉酶消化将糖原与其他AMC阳性成分区分开来。利用H & E/AMC染色，富含粘液的腺癌细胞、阿米巴滋养体和真菌菌丝在紫外激发下清晰可见。使用H & E/AMC染色，可以使用单个组织切片获得H & E和PAS样组织学成像。H & E/AMC可用于病理诊断，特别是当PAS染色的信息至关重要，组织切片数量有限和/或病变较小时。
An aqueous 7-amino-4-methylcoumarin (AMC) solution exhibits strong fluorescence under ultraviolet (UV) light and can be used as a Schiff reagent to visualize aldehydes. We investigated hemalum and eosin (H & E) and AMC staining for histological and pathological analysis. Sections of normal and lesioned human tissues were stained with combined H & E/AMC staining. After H & E/AMC staining, the H & E morphology was preserved under bright field microscopy. The AMC fluorescent signals observed under UV light were intense and the staining pattern was identical to that obtained by periodic acid-Schiff (PAS) staining. AMC staining of archived H & E sections also was successful. Diastase digestion differentiated glycogen from other AMC positive elements. Using H & E/AMC staining, mucus-rich adenocarcinoma cells, amebic trophozoites and fungal hyphae were visualized clearly under UV excitation. Using H & E/AMC staining, H & E and PAS-like histological imaging can be obtained using a single tissue section. H & E/AMC is useful for pathologic diagnosis especially when information from PAS staining is critical, the number of tissue sections is limited and/or the lesion in question is small.