Measuring oxygenation in vivo with MRS/MRI--from gas exchange to the cell.
Measuring oxygenation in vivo with MRS/MRI--from gas exchange to the cell.
复制标题
使用 MRS/MRI 测量体内氧合——从气体交换到细胞。
DOI：
10.1089/ars.2007.1625
复制
发表时间：
2007
影响因子：
6.6
通讯作者：
Dunn,JF
中科院分区：
文献类型：
--
作者：
Dunn,JF
氧在许多信号传导途径、氧化还原代谢和自由基代谢的代谢过程中作为底物发挥重要作用。为了研究氧在正常和病理生理状态下的作用，需要可以无创使用的方法。本文综述了核磁共振技术研究组织氧合的潜力。它是从系统的角度写的，着眼于检测方法，相对于氧气在哺乳动物系统中的路径-从肺，通过血管系统，进入间隙空间，最后进入细胞。讨论的方法范围从那些是可量化的，如评估的自旋晶格弛豫时间在氟碳化合物的解决方案，那些更相关的，如评估的乳酸盐和高能磷酸盐。由于不同的方法在其网站上的应用，灵敏度和特异性的定量氧，这篇评论提供了每种方法已被应用的例子。这可能有助于读者理解如何最佳地应用不同的方法来研究特定的生物医学问题。
Oxygen plays a major role as a substrate in metabolic processes in numerous signaling pathways, in redox metabolism, and in free radical metabolism. To study the role of oxygen in normal and pathophysiological states, methods that can be used noninvasively are required. This review examines the potential of nuclear magnetic resonance techniques to study tissue oxygenation. It is written from a systems perspective, looking at detection methods with respect to the path that oxygen takes in the mammalian system—from the lungs, through the vascular system, into the interstitial space, and finally into the cell. Methods discussed range from those that are quantifiable, such as the assessment of spin lattice relaxation time in fluorocarbon solutions, to those that are more correlative, such as assessment of lactate and high energy phosphates. Since the methods vary in their site of application, sensitivity, and specificity to the quantification of oxygen, this review provides examples of how each method has been applied. This may facilitate the reader's understanding of how to optimally apply different methods to study specific biomedical problems.