The sequential generation of neutrophil chemoattractant proteins in acute inflammation in the rabbit in vivo. Relationship between C5a and proteins with the characteristics of IL-8/neutrophil-activating protein 1.
The sequential generation of neutrophil chemoattractant proteins in acute inflammation in the rabbit in vivo. Relationship between C5a and proteins with the characteristics of IL-8/neutrophil-activating protein 1.
复制标题
兔体内急性炎症中中性粒细胞趋化蛋白的连续产生。
作者：
P. Collins;P. J. Jose;T. J. Williams
以酵母多糖为炎症刺激物，在家兔体内建立实验性腹膜炎模型。在腹膜内注射酵母聚糖后，每隔一段时间取出渗出物，并在试验兔的背部皮肤中进行试验。试验兔接受125 I-白蛋白和111 In-中性粒细胞的静脉注射，并测量每种标记物的局部蓄积，以响应皮内注射渗液样品与增强剂量的PGE 2混合。当在特异性抗C5 a抗体的存在下测试腹膜渗出液样品时，在酵母聚糖注射后2小时内采集的样品中几乎所有的水肿诱导和中性粒细胞化学引诱物活性都被废除。然而，后来的样品含有越来越多的非C5 a成分。在C5 a耗尽6小时渗出液中，使用阳离子交换HPLC分离两个炎症活性峰。有证据表明，C5 a本身是无法刺激这些活动的生产。两个活性峰似乎与IL-8/NAP-1相关，因为它们抑制125 I-IL-8/NAP-1与人嗜中性粒细胞的结合。
An in vivo experimental peritonitis model was investigated in the rabbit using zymosan as the inflammatory stimulus. After an i.p. injection of zymosan, exudate was removed at intervals and tested in the back skin of assay rabbits. Assay rabbits received i.v. injections of 125I-albumin and 111In-neutrophils, and the local accumulation of each label was measured in response to intradermal injections of exudate samples mixed with a potentiating dose of PGE2. When peritoneal exudate samples were tested in the presence of a specific anti-C5a antibody, virtually all the edema-inducing and neutrophil chemoattractant activity was abolished in samples taken up to 2 h after the zymosan injection. Later samples, however, contained increasing levels of a non-C5a component. In C5a-depleted 6-h exudate two peaks of inflammatory activity were separated using cation exchange HPLC. Evidence is presented that C5a itself is unable to stimulate the production of these activities. Both peaks of activity appear related to IL-8/NAP-1 as they inhibited the binding of 125I-IL-8/NAP-1 to human neutrophils.