SHALLOT-LIKE1 Is a KANADI Transcription Factor That Modulates Rice Leaf Rolling by Regulating Leaf Abaxial Cell Development
SHALLOT-LIKE1 Is a KANADI Transcription Factor That Modulates Rice Leaf Rolling by Regulating Leaf Abaxial Cell Development
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SHALLOT-LIKE1 是一种 KANADI 转录因子，通过调节叶片远轴细胞发育来调节水稻卷叶
DOI：
10.1105/tpc.108.061457
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发表时间：
2009-03-01
期刊：
影响因子：
11.6
通讯作者：
Xue, Hong-Wei
中科院分区：
文献类型：
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作者：
Zhang, Guang-Heng;Xu, Qian;Xue, Hong-Wei
水稻卷叶现象作为重要的农艺性状，备受植物生物学家和育种家的关注。适度的卷叶可以增加品种的光合作用，从而提高谷物产量。然而，相关的分子机制仍不清楚。在这里，我们展示了控制水稻卷叶的关键基因 SHALLOT-LIKE1 (SLL1) 的分离和功能表征。 sll1 突变体植物由于背面厚壁细胞发育缺陷而具有极度内弯的叶子。 SLL1 的表达可以在功能上挽救发育缺陷。 SLL1 在多种组织中转录，并在叶子发育过程中积累在远轴表皮中。 SLL1 编码属于 KANADI 家族的 SHAQKYF 类 MYB 家族转录因子。 SLL1缺陷导致远轴叶肉细胞有缺陷的程序性细胞死亡并抑制远轴特征的发育。相比之下，增强的SLL1表达会刺激远轴侧的韧皮部发育，并抑制近轴侧的泡状细胞和厚壁组织发育。此外，SLL1 缺乏会导致叶绿素和光合作用增加。我们的研究结果确定了 SLL1 在调节叶片远轴细胞发育和维持叶片发育过程中远轴特征中的作用。这些结果应该有助于尝试利用分子育种通过调节叶片发育和滚动来提高水稻和其他作物的光合能力。
As an important agronomic trait, rice (Oryza sativa L.) leaf rolling has attracted much attention from plant biologists and breeders. Moderate leaf rolling increases the photosynthesis of cultivars and hence raises grain yield. However, the relevant molecular mechanism remains unclear. Here, we show the isolation and functional characterization of SHALLOT-LIKE1 (SLL1), a key gene controlling rice leaf rolling. sll1 mutant plants have extremely incurved leaves due to the defective development of sclerenchymatous cells on the abaxial side. Defective development can be functionally rescued by expression of SLL1. SLL1 is transcribed in various tissues and accumulates in the abaxial epidermis throughout leaf development. SLL1 encodes a SHAQKYF class MYB family transcription factor belonging to the KANADI family. SLL1 deficiency leads to defective programmed cell death of abaxial mesophyll cells and suppresses the development of abaxial features. By contrast, enhanced SLL1 expression stimulates phloem development on the abaxial side and suppresses bulliform cell and sclerenchyma development on the adaxial side. Additionally, SLL1 deficiency results in increased chlorophyll and photosynthesis. Our findings identify the role of SLL1 in the modulation of leaf abaxial cell development and in sustaining abaxial characteristics during leaf development. These results should facilitate attempts to use molecular breeding to increase the photosynthetic capacity of rice, as well as other crops, by modulating leaf development and rolling.