Identification, cloning, and developmental expression of hepatoma-derived growth factor in the developing rat heart
Identification, cloning, and developmental expression of hepatoma-derived growth factor in the developing rat heart
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DOI：
10.1002/dvdy.1204
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发表时间：
2001-11-01
影响因子：
2.5
通讯作者：
Everett, AD
中科院分区：
文献类型：
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作者：
Everett, AD
利用12天大鼠胎儿圆锥动脉干和新生大鼠主动脉的mRNA差异显示技术，将肝癌衍生生长因子（HDGF）鉴定为一个发育调控的心脏基因。从大鼠胎心cDNA文库中克隆了全长大鼠HDGF cDNA，发现与小鼠和人的序列分别具有94%和88%的同源性。大鼠的序列，像人类和小鼠，包含一个高度保守的氨基部分和假定的二分核定位序列。通过北方分析，RDGF在胎儿圆锥干、心脏、肾脏、脑和肠道中高度表达。通过免疫细胞化学，HDGF首先仅在E10大鼠的心房肌细胞、后肠上皮细胞和脊索中检测到，具有核表达模式。到E12，表达范围扩大到包括心室肌细胞、内皮细胞和心室流出道细胞。HDGF的独特之处在于它是第一个描述的发育中心脏的核靶向生长因子。HDGF在胚胎心脏和胎儿肠道中的早期表达提示HDGF可能在心血管的生长和分化中起作用。(C)2001 Wiley-Liss，Inc.
Hepatoma derived growth factor (HDGF) was identified as a developmentally regulated cardiac gene by mRNA differential display using 12-day rat fetal conotruncus vs. newborn aorta. The full-length rat HDGF cDNA was cloned from a rat fetal heart cDNA library and found to be 94 and 88% homologous to the mouse and human sequence, respectively. The rat sequence, like the human and mouse, contains a highly conserved amino portion and putative bipartite nuclear localization sequence. By Northern analysis, RDGF is highly expressed in the fetal conotruncus, heart, kidney, brain, and gut. By immunocytochemistry, HDGF was first detected only in atrial myocytes, hind gut epithelia, and notochord of the E10 rat with a nuclear expression pattern. By E12, expression had broadened to include the ventricular myocytes, endocardial cells, and cells of the ventricular outflow tract. HDGF is unique in that it is the first described nuclear targeted growth factor in the developing heart. The early expression of HDGF in embryonic heart and fetal gut suggests that HDGF may play a role in cardiovascular growth and differentiation. (C) 2001 Wiley-Liss, Inc.