IMMUNOCHEMICAL DIVERSITY OF THE MAJOR OUTER-MEMBRANE PROTEIN OF AVIAN AND MAMMALIAN CHLAMYDIA-PSITTACI
IMMUNOCHEMICAL DIVERSITY OF THE MAJOR OUTER-MEMBRANE PROTEIN OF AVIAN AND MAMMALIAN CHLAMYDIA-PSITTACI
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DOI：
10.1128/jcm.26.4.675-680.1988
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发表时间：
1988-04-01
影响因子：
9.4
通讯作者：
HIRAI, K
中科院分区：
文献类型：
--
作者：
FUKUSHI, H;HIRAI, K
采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和兔超免疫抗血清免疫印迹法，比较了从鹦鹉、火鸡、鸽子、火鸡、人、猫、麝鼠、羊、牛和沙眼衣原体L2/434/Bu分离的16株鹦鹉、火鸡、猫和牛源鹦鹉衣原体主要外膜蛋白（MOMP）的免疫化学性质。菌株的MOMPs在分子量和免疫特异性上存在差异。根据MOMP的免疫特异性，将15株鹦鹉螺菌株分为禽型和哺乳类两种，另1株未进行分类。免疫印迹法基于MOMP的特异性进行免疫学分类是一种有价值的分类方法。
Immunochemical properties of the major outer membrane protein (MOMP) of 16 strains of Chlamydia psittaci isolated from psittacine birds, budgerigars, a pigeon, turkeys, humans, cats, a muskrat, sheep, and cattle and a strain of C. trachomatis, L2/434/Bu, were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by immunoblotting analysis with hyperimmunized rabbit antisera to strains of parrot, turkey, feline, and bovine origin. The MOMPs of the strains showed variation in molecular weights and immunological specificities. Fifteen of the C. psittaci strains were classified into two avian and two mammalian types based on immunological specificity of the MOMP, whereas the other strain was not classified in this study. Immunological classification based on specificity of the MOMP by immunoblotting proved to be a valuable method to classify various strains of C. psittaci.