Fiber type composition and maximum shortening velocity of muscles crossing the human shoulder
Fiber type composition and maximum shortening velocity of muscles crossing the human shoulder
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DOI：
10.1002/ca.20349
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发表时间：
2007-03-01
期刊：
影响因子：
2.4
通讯作者：
Hughes, R. E.
中科院分区：
文献类型：
--
作者：
Srinivasan, R. C.;Lungren, M. P.;Hughes, R. E.
对14块跨越人盂肱关节的肌肉的纤维类型组成进行了研究，目的是确定纤维类型对整个肌肉横截面积（CSA）的贡献，并估计这些肌肉的最大缩短速度（V-max）。在死亡后24小时内，从4具男性尸体（平均年龄50岁）中获取肌肉活检组织，快速冷冻，固定并横切（10 μ m）。在碱性预孵育后，对载玻片进行肌原纤维ATP酶染色。使用Bioquant系统拍摄限定区域（100根纤维）的照片图像，并从这些图像测量纤维类型和CSA。mATPase染色产生三种不同的纤维类型：慢氧化（SO），快氧化糖酵解（FOG），和快糖酵解（FG）。平均而言，肌纤维类型的组成范围从22至40%的FG，从17至51%的FOG，从23至56%的SO。14块肌肉中有12块的平均SO比例为35%至50%。V-max是根据文献中纤维类型相对于CSA的贡献和缩短速度值计算的。本文提出的最大缩短速度为开发人类肩部肌肉骨骼模型提供了生理基础，该模型适用于预测功能相关任务（包括肌肉缩短和延长条件）的肌肉力。
A study of the fiber type composition of fourteen muscles spanning the human glenohumeral joint was carried out with the purpose of determining the contribution of fiber types to overall muscle cross-sectional area (CSA) and to estimate the maximum shortening velocity (V-max) of those muscles. Muscle biopsies were procured from 4 male cadavers (mean age 50) within 24 hr of death, snap frozen, mounted, and transversely sectioned (10 gym). Slides were stained for myofibrillar ATPase after alkaline preincubation. Photoimages were taken of defined areas (100 fibers) using the Bioquant system, and fiber type and CSA were measured from these images. Staining for mATPase produced three different fiber types: slow-oxidative (SO), fast-oxidative-glycolytic (FOG), and fast-glycolytic (FG). On average, the muscle fiber type composition ranged from 22 to 40% of FG, from 17 to 51% of FOG, and from 23 to 56% of SO. Twelve out of the 14 muscles had average SO proportions ranging from 35 to 50%. V-max was calculated from the fiber type contribution relative to CSA and shortening velocity values taken from the literature. The maximum velocities of shortening presented here provide a physiological basis for the development of human shoulder musculoskeletal models suitable for predicting muscle forces for functionally relevant tasks encompassing conditions of muscle shortening and lengthening.