Line-scanning particle image velocimetry: an optical approach for quantifying a wide range of blood flow speeds in live animals.
Line-scanning particle image velocimetry: an optical approach for quantifying a wide range of blood flow speeds in live animals.
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DOI：
10.1371/journal.pone.0038590
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Wang RA
中科院分区：
文献类型：
--
作者：
Kim TN;Goodwill PW;Chen Y;Conolly SM;Schaffer CB;Liepmann D;Wang RA
测量血液速度的能力对于研究血管发育、生理学和病理学是至关重要的。一个关键的挑战是量化范围广泛的血液速度在血管深处的活标本与并发衍射有限分辨率成像的血管细胞。双光子激光扫描显微镜（TPLSM）在以细胞分辨率分析动物体内数百微米深处的血液速度方面显示出巨大的前景。然而，目前对基于TPLSM的数据的分析仅限于较低范围的血流速度，不足以研究许多正常或疾病条件下的更快速度。我们开发了线扫描粒子图像测速仪（LS-PIV），它使用TPLSM数据来量化峰值血流速度高达84 mm/s的活小鼠窝藏脑动静脉畸形，一种疾病的特点是高流量。通过这种方法，我们能够准确地检测到这些动物中沿着异常血管网络的血液速度升高和脉动性增强沿着。LS-PIV强大地分析了来自脑表面以下850 µm深处血管的噪声数据。除了分析体内数据外，我们还使用已知速度和噪声参数的模拟验证了LS-PIV的精度高达800 mm/s。据我们所知，这些血流速度测量是用TPLSM记录的最快的。与携带细胞特异性荧光报告基因的转基因小鼠合作，LS-PIV还将使高流量血管发育和疾病（如动脉粥样硬化形成、动脉形成和血管异常）中的细胞、生化和血液动力学参数在体内直接相关。
The ability to measure blood velocities is critical for studying vascular development, physiology, and pathology. A key challenge is to quantify a wide range of blood velocities in vessels deep within living specimens with concurrent diffraction-limited resolution imaging of vascular cells. Two-photon laser scanning microscopy (TPLSM) has shown tremendous promise in analyzing blood velocities hundreds of micrometers deep in animals with cellular resolution. However, current analysis of TPLSM-based data is limited to the lower range of blood velocities and is not adequate to study faster velocities in many normal or disease conditions. We developed line-scanning particle image velocimetry (LS-PIV), which used TPLSM data to quantify peak blood velocities up to 84 mm/s in live mice harboring brain arteriovenous malformation, a disease characterized by high flow. With this method, we were able to accurately detect the elevated blood velocities and exaggerated pulsatility along the abnormal vascular network in these animals. LS-PIV robustly analyzed noisy data from vessels as deep as 850 µm below the brain surface. In addition to analyzing in vivo data, we validated the accuracy of LS-PIV up to 800 mm/s using simulations with known velocity and noise parameters. To our knowledge, these blood velocity measurements are the fastest recorded with TPLSM. Partnered with transgenic mice carrying cell-specific fluorescent reporters, LS-PIV will also enable the direct in vivo correlation of cellular, biochemical, and hemodynamic parameters in high flow vascular development and diseases such as atherogenesis, arteriogenesis, and vascular anomalies.
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影响因子：
1.2
作者：
Drew PJ;Blinder P;Cauwenberghs G;Shih AY;Kleinfeld D
通讯作者：
Kleinfeld D
DOI：
10.1152/ajprenal.00521.2005
发表时间：
2006-08-01
影响因子：
4.2
作者：
Kang, Jung Julie;Toma, Ildiko;Peti-Peterdi, Janos
通讯作者：
Peti-Peterdi, Janos
影响因子：
48
作者：
Farrar, Matthew J.;Bernstein, Ida M.;Schlafer, Donald H.;Cleland, Thomas A.;Fetcho, Joseph R.;Schaffer, Chris B.
通讯作者：
Schaffer, Chris B.
影响因子：
4.6
作者：
Huang, CQ;Sheikh, F;Chen, J
通讯作者：
Chen, J
DOI：
10.1073/pnas.95.26.15741
发表时间：
1998-12-22
影响因子：
11.1
作者：
Kleinfeld, D;Mitra, PP;Denk, W
通讯作者：
Denk, W