Conditions That Increase Latent Infection of Grafted or Non-grafted Tomatoes with Pseudomonas solanacearum
Conditions That Increase Latent Infection of Grafted or Non-grafted Tomatoes with Pseudomonas solanacearum
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增加嫁接或非嫁接番茄青枯假单胞菌潜伏感染的条件
DOI：
10.3186/jjphytopath.62.234
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Y. Sumida
中科院分区：
文献类型：
--
作者：
K. Nakaho;S. Takaya;Y. Sumida
用青枯假单胞菌（Pseudomonassolanacearum）接种感病番茄品种Ponderosa和抗病砧木LS-89和BF-Okitsu 101、嫁接在Ponderosa或LS-89砧木上的Ponderosa或LS-89接穗的幼苗根部。在接种后14天，对幼苗进行疾病严重程度指数化，并收获以从茎组织中回收细菌病原体。许多抗病和感病幼苗没有表现出萎蔫症状，但被青枯病菌潜伏感染。随着接种时植株年龄的增加，接种物浓度或培养温度的降低，潜伏感染和萎蔫的频率降低。在LS-89中，在25/20°C（白天/夜晚）下生长的植物几乎没有表现出症状，但具有20- 75%的潜伏感染。对5个青枯病菌株系，在黄松和LS-89中的萎蔫率和潜伏侵染率无显著差异，而在BF-冲津101中的萎蔫率和潜伏侵染率差异显著。黄松和LS-89接穗嫁接黄松几乎都枯萎了。然而，黄松接穗嫁接在LS-89上枯萎了近60%，而LS-89接穗嫁接在LS-89上没有表现出枯萎。69%的无菌LS-89接穗和35%的无菌黄松接穗确实有潜伏感染。这些结果表明，抗病砧木嫁接感病接穗的青枯病是由于病原菌从潜伏感染的砧木转移到接穗。
Seedling roots of the susceptible tomato cultivar Ponderosa and the resistant rootstock cultivars LS-89 and BF-Okitsu 101, Ponderosa or LS-89 scions grafted on Ponderosa or LS-89 rootstocks were inoculated with Pseudomonas solanacearum. At 14 days after inoculation, seedlings were indexed for disease severity and harvested to recover the bacterial pathogen from stem tissues. Many of the resistant and susceptible seedlings did not show wilting symptoms but were latently infected with P. solanacearum. As plant age at the time of inoculation increased, and inoculum concentration or incubation temperature decreased, the frequency of latent infection and wilting decreased. In LS-89, plants grown at 25/20°C (day/night) showed almost no symptoms but had latent infection at 20-75%. Against five strains of P. solanacearum, the percentage of wilting and latent infection of seedlings was not different in Ponderosa or LS-89, but it was significantly different in BF-Okitsu 101. Both Ponderosa and LS-89 scions grafted on Ponderosa had nearly all wilted. However, Ponderosa scions gratfed on LS-89 wilted at nearly 60%, whereas LS-89 scions grafted on LS-89 showed no wilting. Sixty-nine percent of these symptomless LS-89 scions and 35% of the symptomless Ponderosa scions did have latent infections. These results indicated that bacterial wilt of susceptible scions grafted on resistant rootstocks was due to a movement of the pathogen from latently infected rootstocks into the scions.