Characterization of the aldehyde reactive probe reaction with AP-sites in DNA: Influence of AP-lyase on adduct stability
Characterization of the aldehyde reactive probe reaction with AP-sites in DNA: Influence of AP-lyase on adduct stability
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DOI：
10.1080/15257770600726133
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发表时间：
2006-01-01
影响因子：
1.3
通讯作者：
Kitner, Joshua
中科院分区：
文献类型：
--
作者：
Bennett, Samuel E.;Kitner, Joshua
烷氧基胺与DNA中AP位点的开链醛形式反应以产生开链醛肟。在这里，我们的特点的AP-位点裂解酵母AP-内切核酸酶1（APN 1）或T4嘧啶二聚体DNA糖基化酶/AP-裂解酶（T4 Pdg）的效率和稳定性的烷氧基胺醛反应性探针（APLP）缩合反应与AP-网站的效果。结果表明：（1）APLP与AP-位点的开链醛平衡形式的反应效率低于与T4 Pdg产生的3 '-α，β-不饱和醛的反应效率;（3）AP-位点和3 ′-α，β-不饱和醛在37 ℃下与APLP反应30分钟是稳定的，和（4）APLP加合成AP-β-不饱和醛的开链醛形式。位点可被甲氧基胺取代，但3 '-α，β-不饱和醛APLP肟对甲氧基胺攻击稳定。
Alkoxyamines react with the open-chain aldehyde form of AP-sites in DNA to produce open-chain aldehyde oximes. Here we characterize the effect of AP-site cleavage by yeast AP-endonuclease 1 (APN1) or T4 pyrimidine dimer DNA glycosylase/AP-lyase (T4 Pdg) on the efficiency and stability of the alkoxyamine aldehyde reactive probe (APLP) condensation reaction with AP-sites. The results indicate that (1) reaction of APLP with the open-chain aldehyde equilibrium form of the AP-site was less efficient than with the 3'-alpha,beta-unsaturated aldehyde produced by T4 Pdg; (2) the dRP moiety was least reactive with ARP; (3) both the AP-site and 3'-alpha,beta-unsaturated aldehyde were stable with regard to reaction with APLP over a 30-min incubation period at 37 degrees C and (4) APLP adducted to the open-chain aldehyde form of the AP-site could be replaced by methoxyamine, but the 3'-alpha,beta-unsaturated aldehyde APLP oxime was stable against methoxyamine attack.