SITES OF REPLICATION OF A PORCINE RESPIRATORY CORONAVIRUS RELATED TO TRANSMISSIBLE GASTROENTERITIS VIRUS
SITES OF REPLICATION OF A PORCINE RESPIRATORY CORONAVIRUS RELATED TO TRANSMISSIBLE GASTROENTERITIS VIRUS
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DOI：
10.1016/s0034-5288(18)30984-6
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发表时间：
1990-03-01
影响因子：
2.4
通讯作者：
PENSAERT, MB
中科院分区：
文献类型：
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作者：
COX, E;HOOYBERGHS, J;PENSAERT, MB
用气雾剂将猪呼吸道冠状病毒(PRCV)接种于9头1周龄子宫切除和剥夺初乳的仔猪。接种后不同时间处死动物，取组织标本进行病毒分离和免疫荧光检测。结果表明，病毒在呼吸道内以高滴度复制。复制主要见于肺泡细胞，也见于鼻黏膜、气管、支气管、细支气管处的上皮细胞、肺泡巨噬细胞和扁桃体。在呼吸道进行初次复制后，出现病毒血症。病毒吞咽后也到达了胃肠道。随后，观察到PRCV在回肠中复制。感染在几天内从回肠扩散到十二指肠。在小肠中的复制仍然局限于位于绒毛和或隐窝的上皮层或下面的少数细胞。无法确定细胞类型。从所有猪的肠系膜淋巴结中分离到病毒，但没有观察到免疫荧光。结果表明，传染性胃肠炎病毒和新城疫病毒之间分子结构的微小变化导致宿主细胞嗜性的重要变化。
A porcine respiratory coronavirus (PRCV) was inoculated by aerosol into nine hysterectomy-derived and colostrum-deprived pigs at the age of one week. They were killed at different times after inoculation and tissues were sampled for virus isolation and immunofluorescence. Results indicate that virus replicated to high titres in the respiratory tract. Replication mainly occurred in alveolar cells but also in epithelial cells of nasal mucosa, trachea, bronchi, bronchioli, in alveolar macrophages and in tonsils. After primary replication in the respiratory tract, viraemia occurred. Virus also reached the gastrointestinal tract after swallowing. Subsequently, PRCV was observed to replicate in the ileum. The infection spread within a few days from the ileum to the duodenum. Replication in the small intestine remained limited to a few cells located in or underneath the epithelial layer of villi and, or, crypts. The cell type could not be identified. Virus was isolated from mesenteric lymph nodes in all pigs, but immunofluorescence was not observed. Results show that small changes in molecular structure between transmissible gastroenteritis virus and PRCV resulted in important changes in host cell tropism.