Field-effect sensors with charged macromolecules:: Characterisation by capacitance-voltage, constant-capacitance, impedance spectroscopy and atomic-force microscopy methods
Field-effect sensors with charged macromolecules:: Characterisation by capacitance-voltage, constant-capacitance, impedance spectroscopy and atomic-force microscopy methods
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DOI：
10.1016/j.bios.2006.09.014
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发表时间：
2007-04-15
影响因子：
12.6
通讯作者：
Schoning, M. J.
中科院分区：
文献类型：
--
作者：
Poghossian, A.;Abouzar, M. H.;Schoning, M. J.
场效应型电容式电解质-绝缘体-半导体(EIS)传感器已被用于脱氧核糖核酸(DNA)的固定和杂化检测，以及监测聚电解质(阴离子聚4-苯磺酸钠(PSS)和阳离子聚烯丙基胺盐酸盐(PAH))的逐层吸附。用电容-电压法、恒电容法、阻抗谱和原子力显微镜等方法对带电大分子EIS传感器进行了系统的表征。研究了聚电解质层的数目和极性对电容-电压曲线移动的影响。每个聚阴离子和聚阳离子层分别吸附后，观察到约30-90 mV的交流电位漂移。DNA固定化信号为35-55 mV，杂交信号为24-33 mV。讨论了传感器响应的可能机制。(C)2006爱思唯尔B.V.保留所有权利。
Field-effect-based capacitive electrolyte-insulator-semiconductor (EIS) sensors have been utilised for the deoxyribonucleic acid (DNA) immobilisation and hybridisation detection as well as for monitoring the layer-by-layer adsorption of polyelectrolytes (anionic poly(sodium 4-styrene sulfonate) (PSS) and cationic poly(allylamine hydrochloride) (PAH)). The EIS sensors with charged macromolecules have been systematically characterised by capacitance-voltage, constant-capacitance, impedance spectroscopy and atomic-force microscopy methods. The effect of the number and polarity of the polyelectrolyte layers on the shift of the capacitance-voltage curves has been investigated. Alternating potential shifts of about 30-90 mV have been observed after the adsorption of each polyanion and polycation layer, respectively. The DNA immobilisation and hybridisation signals were 35-55 and 24-33 mV, respectively. The possible mechanisms for the sensor responses are discussed. (c) 2006 Elsevier B.V. All rights reserved.