Pre-clinical characterization of tissue engineering constructs for bone and cartilage regeneration.
Pre-clinical characterization of tissue engineering constructs for bone and cartilage regeneration.
复制标题
用于骨和软骨再生的组织工程结构的临床前表征。
DOI：
10.1007/s10439-014-1151-0
复制
发表时间：
2015
影响因子：
3.8
通讯作者：
Mikos,AntoniosG
中科院分区：
文献类型：
--
作者：
Trachtenberg,JordanE;Vo,TiffanyN;Mikos,AntoniosG
临床前动物模型在生物医学技术从实验室到临床的转化中起着至关重要的作用。然而，需要改进技术来评价宿主内植入的生物材料，包括考虑与动物研究相关的护理和伦理，以及以临床相关方式评价宿主组织修复。这篇综述讨论了非侵入性，定量和实时技术，用于评估宿主材料的相互作用，新组织形成的质量和速度，以及植入骨和软骨组织工程生物材料的功能结果。具体而言，将对临床前动物模型、组织学评分系统和非侵入性成像方式进行比较。此外，将讨论跟踪递送的细胞和生长因子的新技术，包括将其释放与组织生长直接相关的方法。
Pre-clinical animal models play a crucial role in the translation of biomedical technologies from the bench top to the bedside. However, there is a need for improved techniques to evaluate implanted biomaterials within the host, including consideration of the care and ethics associated with animal studies, as well as the evaluation of host tissue repair in a clinically relevant manner. This review discusses non-invasive, quantitative, and real-time techniques for evaluating host-materials interactions, quality and rate of neotissue formation, and functional outcomes of implanted biomaterials for bone and cartilage tissue engineering. Specifically, a comparison will be presented for pre-clinical animal models, histological scoring systems, and non-invasive imaging modalities. Additionally, novel technologies to track delivered cells and growth factors will be discussed, including methods to directly correlate their release with tissue growth.