Comparison of RNA expression profiles between the two generations of Porphyra yezoensis (Rhodophyta), based on expressed sequence tag frequency analysis
Comparison of RNA expression profiles between the two generations of Porphyra yezoensis (Rhodophyta), based on expressed sequence tag frequency analysis
复制标题
DOI：
10.1046/j.1529-8817.2003.03003.x
复制
发表时间：
2003-10-01
影响因子：
2.9
通讯作者：
Tabata, S
中科院分区：
文献类型：
--
作者：
Asamizu, E;Nakajima, M;Tabata, S
为筛选与条斑紫菜（Porphyra yezoensis上田）配子体和孢子体世代形态和生理差异相关的候选基因，进行了大规模表达序列标签（EST）分析。从孢子体cDNA文库中总共产生了10，625个5/-末端EST序列，除了先前公布的10，154个来自配子体的EST之外。以50个碱基的95%同源性为标准，将20,779个EST聚为4496个非冗余组。在EST组中，只有1013（22.5%）组被归类为在两个世代中常见的EST，而很大比例的EST组被确定为是独特的配子体（1940 EST组= 43.1%）或孢子体（1543 EST组= 34.3%）。统计学显著性检验显示，89和112高表达的候选基因的配子体和孢子体，分别。对12个候选基因进行RT-PCR分析，证实差异表达。通过RT-PCR分析证明了转录物的选择性剪接的世代特异性调节。
To identify gene candidates related to the morphological and physiological differences between the gametophytic and sporophytic generations of a marine red alga, Porphyra yezoensis Ueda, large-scale expressed sequence tag (EST) analysis was conducted. A total of 10,625 5/-end EST sequences was generated from the sporophyte cDNA library, in addition to the previously published 10,154 ESTs from the gametophyte. A total of 20,779 ESTs was clustered into 4496 nonredundant groups at the criterion of 95% identity for 50 bases. Among the EST groups, only 1013 (22.5%) groups were classified as ESTs that commonly occurred in both generations, whereas a large proportion of EST groups were identified as being unique to either the gametophyte (1940 EST groups = 43.1%) or the sporophyte (1543 EST groups = 34.3%). A statistical significance test revealed 89 and 112 highly expressed gene candidates in the gametophyte and the sporophyte, respectively. Twelve candidate genes were subjected to RT-PCR analysis, and the differential expression was confirmed. Generation-specific regulation of alternative splicing of a transcript was demonstrated by RT-PCR analyses.