IDENTIFICATION OF RAPD MARKERS LINKED TO A GENE GOVERNING CADMIUM UPTAKE IN DURUM-WHEAT
IDENTIFICATION OF RAPD MARKERS LINKED TO A GENE GOVERNING CADMIUM UPTAKE IN DURUM-WHEAT
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作者：
PENNER, GA;CLARKE, J;LEISLE, D
采用温度扫描凝胶电泳法和随机扩增多态性DNA分析相结合的方法，对加拿大西部硬粒小麦L低镉吸收单基因的两个标记进行了检测。瓦尔硬脑膜)。对高镉积累量品种Kyle与低镉积累量品种尼罗河杂交后代进行分析，得到4.6(OPC-20)和21.2(UBC-180)cM的连锁估计。最近的标记(OPC-20)被证明对从另外两个低镉来源‘Biodur’和‘Hercules’中进行低镉吸收选择是有用的。利用该标记对20个引进硬粒小麦品系的抗性遗传基础进行了分析。在这个不同的种质范围内，除了两个案例外，该标记与镉含量的预期都是相关的。镉含量的化学测定成本较高，阻碍了植物低镉品种的选育。低镉吸收基因的标记辅助选择提供了一种有效的替代方法。
Temperature sweep gel electrophoresis in combination with random amplified polymorphic DNA analysis was employed to detect two markers for a single gene governing low cadmium uptake in western Canadian durum wheat (Triticum turgidum L,. var. durum). Analysis of progeny derived from a cross of the high cadmium accumulating cultivar Kyle by the low cadmium accumulating cultivar Nile resulted in linkage estimates of 4.6 (OPC-20) and 21.2 (UBC-180) cM. The closest marker (OPC-20) was shown to be useful for making low cadmium uptake selections from two other sources of low cadmium,'Biodur' and 'Hercules'. The marker was further used to determine the genetic basis of resistance in 20 introduced durum wheat lines. Within this diverse range of germplasm the marker was correlated with cadmium contents as expected in all but two cases. Plant breeding selection for low cadmium genotypes is hindered by the high cost of chemical determination of cadmium content. Marker assisted selection for a low cadmium uptake gene offers an effective alternative.