Epitope Binning of Monoclonal and Polyclonal Antibodies by Biolayer Interferometry.
Epitope Binning of Monoclonal and Polyclonal Antibodies by Biolayer Interferometry.
复制标题
通过生物层干涉测量法对单克隆和多克隆抗体进行表位分类。
DOI：
10.1007/978-1-0716-3239-0_2
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Mousa,JarrodJ
中科院分区：
文献类型：
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作者：
Nagashima,Kaito;Mousa,JarrodJ
了解由感染和疫苗接种引起的抗体的表位通常在免疫原设计中是有用的。在本章中，我们描述了基于生物层干涉（BLI）的方法来评估这些表位，并允许同时分析来自几个来源的抗体，包括单克隆抗体（mAb）和多克隆血清抗体（pAb）。使用先前表征的具有已知表位的抗体作为对照，显示了分离的人mAb和来自HA免疫小鼠的合并血清的流感血凝素（HA）的表位分布。该方法是通用的，高通量的，并且可以适应于多种抗原。
Understanding the epitopes of antibodies elicited by infection and vaccination is often useful in immunogen design. In this chapter, we describe biolayer interferometry (BLI)-based methods to evaluate such epitopes and permit simultaneous analysis of antibodies from several sources, including monoclonal antibodies (mAbs) and polyclonal serum antibodies (pAbs). Using previously characterized antibodies with known epitopes as controls, the distribution of epitopes for the influenza hemagglutinin (HA) is shown for isolated human mAbs and pooled serum from HA-immunized mice. This method is versatile, high-throughput, and can be adapted to several antigens.