A molecular imprinting-based turn-on Ratiometric fluorescence sensor for highly selective and sensitive detection of 2,4-dichlorophenoxyacetic acid (2,4-D)
A molecular imprinting-based turn-on Ratiometric fluorescence sensor for highly selective and sensitive detection of 2,4-dichlorophenoxyacetic acid (2,4-D)
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基于分子印迹的开启比率荧光传感器，用于高选择性和灵敏地检测 2,4-二氯苯氧基乙酸 (2,4-D)
DOI：
10.1016/j.bios.2016.03.031
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发表时间：
2016-07-15
影响因子：
12.6
通讯作者：
Chen, Lingxin
中科院分区：
文献类型：
--
作者：
Wang, Xiaoyan;Yu, Jialuo;Chen, Lingxin
以硝基苯并恶二唑（NBD）为检测信号源，量子点（QD）为参比信号源，通过溶胶-凝胶聚合法，构建了一种基于光诱导电子转移（PET）的2，4-二氯苯氧乙酸（2，4-D）开启率荧光分子印迹传感器。随着2，4-D的存在和增加，QDs@SiO2表面上的胺基可以与2，4-D结合，从而使NBD荧光强度显著增强，因为PET过程受到抑制，而量子点保持恒定的强度。因此，绿色NBD和红色QD的双发射强度的比率可用于2，4-D的开启荧光检测，沿着肉眼容易观察到的从橙红色到绿色的连续颜色变化。所制备的荧光传感器获得了高灵敏度与0.14 μ M的低检测限在5分钟内，和区别识别选择性的2，4-D超过其类似物。将该传感器成功地应用于真实的水样中2，4-D的测定，在3个加标水平下2，4-D的回收率为95.0%~ 110.1%，精密度小于4.5%。这种简单、快速、可靠的视觉传感策略不仅为复杂基体的高选择性超痕量分析提供了潜在的应用前景，而且极大地丰富了分子印迹传感器的研究内涵。(C)2016爱思唯尔B. V.保留所有权利。
A novel molecular imprinting-based turn-on ratiometric fluorescence sensor was constructed via a facile sol-gel polymerization for detection of 2,4-dichlorophenoxyacetic acid (2,4-D) on the basis of photo induced electron transfer (PET) by using nitrobenzoxadiazole (NBD) as detection signal source and quantum dots (QDs) as reference signal source. With the presence and increase of 2,4-D, the amine groups on the surface of QDs@SiO2 could bind with 2,4-D and thereby the NBD fluorescence intensities could be significantly enhanced since the PET process was inhibited, while the QDs maintained constant intensities. Accordingly, the ratio of the dual-emission intensities of green NBD and red QDs could be utilized for turn-on fluorescent detection of 2,4-D, along with continuous color changes from orange-red to green readily observed by the naked eye. The as-prepared fluorescence sensor obtained high sensitivity with a low detection limit of 0.14 mu M within 5 min, and distinguished recognition selectivity for 2,4-D over its analogs. Moreover, the sensor was successfully applied to determine 2,4-D in real water samples, and high recoveries at three spiking levels of 2,4-D ranged from 95.0% to 110.1% with precisions below 4.5%. The simple, rapid and reliable visual sensing strategy would not only provide potential applications for high selective ultratrace analysis of complicated matrices, but also greatly enrich the research connotations of molecularly imprinted sensors. (C) 2016 Elsevier B.V. All rights reserved.