STRUCTURE OF THE L(2)GL GENE OF DROSOPHILA AND DELIMITATION OF ITS TUMOR SUPPRESSOR DOMAIN
STRUCTURE OF THE L(2)GL GENE OF DROSOPHILA AND DELIMITATION OF ITS TUMOR SUPPRESSOR DOMAIN
复制标题
DOI：
10.1016/0092-8674(87)90217-0
复制
发表时间：
1987-07-17
期刊：
影响因子：
64.5
通讯作者：
MECHLER, BM
中科院分区：
文献类型：
--
作者：
JACOB, L;OPPER, M;MECHLER, BM
我们之前克隆了致命（2）巨型幼虫，这是果蝇的一种肿瘤抑制基因，通常控制大脑视觉中心和成象盘的细胞增殖和/或分化。在这里，我们描述了通过基因组和 cDNA 克隆测序确定的 l(2)gl 基因的结构。 cDNA 的结构表明使用了选择性剪接，无论是在 5'' 非翻译外显子中还是在 3'' 编码外显子中。因此，该基因编码两个假定的蛋白质，分别具有 1161 和 708 个氨基酸，即 p127 和 p78，其 C 末端不同。保留p78编码序列完整但删除p127的141个残基的3''-截短的l(2)gl转座子能够抑制l(2)gl缺陷动物中的肿瘤形成。这些结果表明推定的 p78 蛋白可有效控制细胞增殖和/或分化。
We have previously cloned lethal(2) giant larvae, a tumor-suppressor gene of Drosophila that normally controls cell proliferation and/or differentiation in the optic centers of the brain and the imaginal discs. Here we describe the structure of the l(2)gl gene as determined by sequencing genomic and cDNA clones. The structure of the cDNAs indicates the use of alternative splicing, either in the 5'' untranslated exons or in the 3'' coding exons. Thus the gene encodes two putative proteins of 1161 and 708 amino acis, p127 and p78, respectively, differing at their C termini. A 3''-truncated l(2)gl transposon that leaves the coding sequence of p78 intact but deletes 141 residues of p127 was capable of suppressing tumor formation in l(2)gl-deficient animals. These results suggest that the putative p78 protein is effective in controlling cell proliferation and/or differentiation.