Actinotetraose L, a new tetrasaccharide derivative isolated from the mycelia of Amycolatopsis sp HCa1
Actinotetraose L, a new tetrasaccharide derivative isolated from the mycelia of Amycolatopsis sp HCa1
复制标题
Actinotetraose L，一种从无枝酸菌菌丝体中分离出来的新型四糖衍生物。
DOI：
10.1080/10286020.2015.1052413
复制
发表时间：
2015-11-02
影响因子：
1.7
通讯作者：
Ge, Hui-Ming
中科院分区：
文献类型：
--
作者：
Guo, Zhi-Kai;Yan, Wei;Ge, Hui-Ming
从与蚱蜢相关的稀有放线杆菌无枝酸菌菌丝体的甲醇提取物中分离出一种新的四糖衍生物，放线四糖 L (1)。 HCa1。通过结合 1D、2D NMR（HMQC、HMBC、COSY 和 NOESY）和 HR-ESI-MS 分析，阐明了新化合物的结构：O-{3,4-二-O-[(E)-2-乙基-2-丁烯酰基]- β-D-吡喃葡萄糖基-(1 → 2)}-O-{3,4-二-O-[(E)-2-甲基-2-丁烯酰基]-β-D-吡喃葡萄糖基-(1 → 2)}-6,6-二-O-[(E)-2-甲基-2-丁烯酰基]-α-D-吡喃葡萄糖基-(1 → 1)-α-D-吡喃葡萄糖苷。通过 T 细胞活力和 MTT 测定评估该化合物的免疫抑制活性和细胞毒性。尽管如此，没有观察到显着的活动。
A new tetrasaccharide derivative, actinotetraose L (1), was isolated from the methanol extract of the mycelia of the grasshopper-associated rare actinobacterium Amycolatopsis sp. HCa1. The structure of the new compound was elucidated by a combination of 1D, 2D NMR (HMQC, HMBC, COSY, and NOESY), and HR-ESI-MS analyses as O-{3,4-di-O-[(E)-2-ethyl-2-butenoyl]- β-D-glucopyranosyl-(1 → 2)}-O-{3,4-di-O-[(E)-2-methyl-2-butenoyl]-β-D-glucopyranosyl-(1 → 2)}-6,6-di-O-[(E)-2-methyl-2-butenoyl]-α-D-glucopyranosyl-(1 → 1)-α-D-glucopyranoside. The immunosuppressive activity and cytotoxicity of the compound were evaluated by T-cell viability and MTT assays. Nonetheless, no significant activity was observed.