LABORATORY INVESTIGATIONS ON THE LOW PATHOGENIC POTENTIAL OF PLESIOMONAS-SHIGELLOIDES
LABORATORY INVESTIGATIONS ON THE LOW PATHOGENIC POTENTIAL OF PLESIOMONAS-SHIGELLOIDES
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DOI：
10.1128/jcm.29.1.148-153.1991
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发表时间：
1991-01-01
影响因子：
9.4
通讯作者：
JANDA, JM
中科院分区：
文献类型：
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作者：
ABBOTT, SL;KOKKA, RP;JANDA, JM
对16株从人肠外和肠道疾病、感染动物和环境中分离得到的志贺氏单胞菌的病原学特性进行了研究。大多数菌株具有与志贺氏菌相似的高细胞电荷和低表面疏水性；此外，o17组菌株与D组志贺氏菌抗血清有反应。然而，与志贺氏菌不同的是，志贺氏杆菌菌株并不普遍结合刚果红，在HEp-2细胞检测中无创，并且不会对Vero细胞产生志贺氏样毒素。在HEp-2， Y1，可能还有Vero细胞上，当在Evan Casamino -酵母菌提取物或Penassay肉汤中生长时，所有16株志贺杆菌菌株都一致产生低水平的细胞溶素。所有16株志贺杆菌在远交系瑞士韦氏小鼠中的50%致死剂量中位数为3.5 × 10(8) CFU(范围为3.2 × 10（7)至bb0.1 × 10(9) CFU）。动物致病性与细胞溶解素表达、拥有大于或等于120-MDa的质粒、蛋白质谱或对补体介导的裂解的抗性无关。分析的菌株没有产生铁载体或热稳定的肠毒素。结果表明，无论其分离位点、表型、血清学或表面特性如何，Plesiomonas属的成员总体上具有较低的致病潜力。
The pathogenic properties of 16 Plesiomonas shigelloides strains recovered from humans with extraintestinal and intestinal illnesses, infected animals, and environmental sources were investigated. Most strains possessed a high cell charge and low surface hydrophobicity analogous to those of Shigella spp.; additionally, serogroup O:17 strains reacted with Shigella group D antisera. However, unlike the shigellae, P. shigelloides strains did not universally bind Congo red, were noninvasive in HEp-2 cell assays, and did not produce a Shiga-like toxin on Vero cells. On HEp-2, Y1, and possibly Vero cells, a low-level cytolysin was consistently produced by all 16 P. shigelloides strains when grown in either Evan Casamino Acids-yeast extract or Penassay broth. The median 50% lethal dose for all 16 P. shigelloides strains in outbred Swiss Webster mice was 3.5 x 10(8) CFU (range, 3.2 x 10(7) to > 1 x 10(9) CFU). Animal pathogenicity did not correlate with cytolysin expression, possession of a greater-than-or-equal-to 120-MDa plasmid, protein profile, or resistance to complement-mediated lysis. No strain analyzed produced siderophores or a heat-stable enterotoxin. The results suggest that members of the genus Plesiomonas have an overall low pathogenic potential, irrespective of the site of isolation or phenotypic, serologic, or surface properties shared with other traditional enteropathogens.