Expression of EphA1 in gastric carcinomas is associated with metastasis and survival
Expression of EphA1 in gastric carcinomas is associated with metastasis and survival
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EphA1 在胃癌中的表达与转移和生存相关。
DOI：
10.3892/or_00001020
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发表时间：
2010-12-01
期刊：
影响因子：
4.2
通讯作者：
Zhou, Xiaojun
中科院分区：
文献类型：
--
作者：
Wang, Jiandong;Dong, Yingchun;Zhou, Xiaojun
Eph亚家族的受体酪氨酸激酶参与了某些癌症的发生和发生。本研究检测了EPHA1在胃癌中的表达，并分析了EPHA1的表达与临床病理参数的关系，以探讨EPHA1在胃癌中的作用。采用实时定量逆转录聚合酶链式反应(RT-PCR)检测EPHA1在胃癌组织及相应正常组织中的表达水平。甲基化特异性聚合酶链式反应(MSP)检测EPHA1启动子区域的CpG岛状态，免疫组织化学染色(IHC)检测EPHA1蛋白的表达。EPHA1转录下调的病例占34%，上调的病例占25%，表达无差异的病例占41%。EPHA1mRNA的表达水平与肿瘤大小(P=0.001)、分期(P=0.001)和淋巴结转移(P=0.011)有关。在所检测的大多数EPHA1下调的癌中都检测到甲基化的EPHA1 DNA。EPHA1蛋白表达与肿瘤侵袭深度(P=0.069)、分期(P<；0.001)和淋巴结转移(P=0.018)有关。生存分析显示，EPHA1表达上调的患者预后比肿瘤表达下调(P=0.005)或表达无差异(P=0.003)的患者差。EPHA1可能在胃癌的侵袭和转移中起作用。EPHA1表达水平可作为判断胃癌预后的指标，EPHA1基因可能为胃癌的治疗提供新的靶点。
Receptor tyrosine kinases of the Eph subfamily are involved in the development and the carcinogenesis of certain cancers. In this study we investigated expression of EphA1 in gastric carcinomas, and analyzed associations between EphA1 expression and clinicopathological parameters to investigate the role of EphA1 in gastric carcinoma. The level of EphA1 transcript expression in gastric carcinoma tissue and corresponding normal tissue was determined by quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR). The status of the CpG island associated with the promoter region of EphA1 was assessed by methylation-specific PCR (MSP), and EphA1 protein expression of was examined by immunohistochemical staining (IHC). Down-regulation of EphA1 transcripts was detected in 34% of the cases, up-regulation in 25% of the cases, and no difference in expression in 41% of the cases. The EphA1 transcript expression level was associated with tumor size (P=0.05), stage (P=0.001), and lymph node metastasis (P=0.011). Methylated EphA1 DNA was detected in most of the carcinomas with EphA1 down-regulation that were examined. EphA1 protein expression was associated with depth of wall invasion (P=0.069), stage (P<0.001), and lymph node metastasis (P=0.018). The survival analysis showed that patients whose tumor exhibited EphA1 up-regulation had a poorer outcome than those whose tumor exhibited down-regulation (P=0.005) or no difference in expression (P=0.003). EphA1 may have roles in invasion and metastasis by gastric carcinoma. The EphA1 expression level is a potential prognostic marker in gastric carcinoma, and the EphA1 gene may provide a novel target of therapy for gastric carcinoma.