Production, Characterization, and Antimicrobial Activity of Mycocin Produced by Debaryomyces hansenii DSMZ70238.
Production, Characterization, and Antimicrobial Activity of Mycocin Produced by Debaryomyces hansenii DSMZ70238.
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DOI：
10.1155/2017/2605382
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发表时间：
2017
影响因子：
3.4
通讯作者：
Ibrahim JAA
中科院分区：
文献类型：
--
作者：
Al-Qaysi SAS;Al-Haideri H;Thabit ZA;Al-Kubaisy WHAA;Ibrahim JAA
本研究的目的是评估汉逊曼氏菌 DSMZ70238 产生的杀伤毒素对几种病原微生物的抗菌活性和潜在的生物控制作用。在这项研究中，研究了 NaCl、pH 值和温度、杀手毒素产生和抗菌活性的影响。结果表明，杀伤毒素在8% NaCl时具有最佳抑制效果，对金黄色葡萄球菌、大肠杆菌、肺炎克雷伯菌、化脓性链球菌、白色念珠菌和新型念珠菌的净区直径分别为20、22、22、21、14和13毫米。在 pH 4.5 时观察到最大的抑制区，对于相同的微生物，抑制区为 16、18、17、18、11 和 12mm。结果还表明，25°C 是对所有目标微生物具有毒素杀灭活性的最佳温度。此外，杀伤毒素活性对所有目标病原真菌均显着抑制菌丝体生长，对红色毛癣菌、链格孢菌、同心毛癣菌和新月弯孢菌的抑制率分别为47.77%、48.88%、52.22%和61.11%。结果表明，汉逊石竹DSMZ70238在8% NaCl浓度、pH 4.5、25℃、72小时的条件下生长速率最高。
The present study was conducted to estimate the antimicrobial activity and the potential biological control of the killer toxin produced by D. hansenii DSMZ70238 against several pathogenic microorganisms. In this study, the effects of NaCl, pH, and temperature, killer toxin production, and antimicrobial activity were studied. The results showed that the optimum inhibitory effect of killer toxin was at 8% NaCl, and the diameters of clear zones were 20, 22, 22, 21, 14, and 13 mm for Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes, Candida albicans, and Candida neoformans, respectively. The largest inhibition zones were observed at pH 4.5 with inhibition zone of 16, 18, 17, 18, 11, and 12 mm for the same microorganisms. The results also showed that 25°C is the optimal temperature for toxin killing activity against all targeted microorganisms. In addition, the activity of killer toxin significantly inhibited the growth of fungal mycelia for all target pathogenic fungi and the percentages of inhibition were 47.77, 48.88, 52.22, and 61.11% for Trichophyton rubrum, Alternaria alternata, Trichophyton concentricum, and Curvularia lunata, respectively. The results showed the highest growth rate of D. hansenii DSMZ70238 under condition of 8% NaCl concentration, pH 4.5, and 25°C for 72 h.