A vast evolutionarily transient translatome contributes to phenotype and fitness
A vast evolutionarily transient translatome contributes to phenotype and fitness
复制标题
DOI：
10.1016/j.cels.2023.04.002
复制
发表时间：
2023-05-17
期刊：
影响因子：
9.3
通讯作者：
Carvunis，Anne-Ruxandra
中科院分区：
文献类型：
--
作者：
Wacholder，Aaron;Parikh，Saurin Bipin;Carvunis，Anne-Ruxandra
翻译是核糖体合成蛋白质的过程。核糖体分析最近揭示了许多以前被认为是非编码的短序列被普遍翻译。为了鉴定这种非规范翻译组中的蛋白质编码基因，我们结合了一个非常敏感的核糖体分析的综合框架，iRibo，以及为短序列量身定制的高功率选择推断。我们为酿酒酵母构建了一个参考翻译组，包括5400个规范和近19000个非规范翻译元素。只有14个非规范元素在可检测的净化选择下进化。缺乏选择特征的翻译元件的代表性子集被证明参与了包括DNA修复、应激反应和转录后调控在内的过程。我们的研究结果表明，大多数翻译元件不是保守的蛋白质编码基因，并通过快速进化的分子机制参与基因型-表型关系。
Translation is the process by which ribosomes synthesize proteins. Ribosome profiling recently revealed that many short sequences previously thought to be noncoding are pervasively translated. To identify protein-coding genes in this noncanonical translatome, we combine an integrative framework for extremely sensitive ribosome profiling analysis, iRibo, with high-powered selection inferences tailored for short sequences. We construct a reference translatome forSaccharomyces cerevisiaecomprising 5,400 canonical and almost 19,000 noncanonical translated elements. Only 14 noncanonical elements were evolving under detectable purifying selection. A representative subset of translated elements lacking signatures of selection demonstrated involvement in processes including DNA repair, stress response, and post-transcriptional regulation. Our results suggest that most translated elements are not conserved protein-coding genes and contribute to genotype-phenotype relationships through fast-evolving molecular mechanisms.