Exclusion of NFYB as candidate gene for congenital splay leg in piglets and radiation hybrid mapping of further five homologous porcine genes from human chromosome 12 (HSA12)
Exclusion of NFYB as candidate gene for congenital splay leg in piglets and radiation hybrid mapping of further five homologous porcine genes from human chromosome 12 (HSA12)
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DOI：
10.1159/000106443
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发表时间：
2007-01-01
影响因子：
1.7
通讯作者：
Maak, S.
中科院分区：
文献类型：
--
作者：
Boettcher, D.;Paul, S.;Maak, S.
根据差异显示和QTL分析的结果，将核转录因子Y β（NFYB）作为仔猪先天性外八字腿的候选基因进行了研究。我们将NFYB定位于猪5号染色体（SSC 5）。通过将来自人类染色体（HSA）12q23.3-> q24.11上相应区域的另外5个猪基因分配到SSC 5和14，我们可以将进化断点从大于10 Mb的间隔限制到小于400 kb。NFYB基因编码区的比较序列分析显示，在健康和八字腿仔猪没有多态性。在猪、人、牛、狗、大鼠和小鼠之间，密码子的种间保守性分别为87%至95%。NFYB在M.股二头肌在健康和八字腿仔猪之间没有差异。然而，健康雄性仔猪的表达显著高于雌性仔猪。我们的结果排除了NFYB作为先天性八字腿的候选基因，但为从SSC 5中选择进一步的候选基因提供了基础。Karger AG，巴塞尔。
Nuclear transcription factor Y, beta (NFYB) was evaluated as candidate gene for congenital splay leg in piglets based on data from differential display and QTL analysis. We mapped NFYB to pig chromosome 5 (SSC5). By assigning further five porcine genes from the corresponding region on human chromosome (HSA) 12q23.3 -> q24.11 to SSC5 and 14 we could confine an evolutionary breakpoint from an interval of more than 10 Mb to less than 400 kb. Comparative sequence analysis of the coding region of NFYB in healthy and splay leg piglets revealed no polymorphism. Inter-species conservation of the codons ranges from 87% to 95% between pig, human, cow, dog, rat and mouse, respectively. The expression of NFYB in M. biceps femoris was not different between healthy and splay leg piglets. However, healthy male piglets had a significantly higher expression than females. Our results exclude NFYB as candidate gene for congenital splay leg but provide a basis for selection of further candidates for the disease from SSC5.Copyright (c) 2007 S. Karger AG, Basel.