Spatio-temporal MAPK dynamics mediate cell behavior coordination during fungal somatic cell fusion
Spatio-temporal MAPK dynamics mediate cell behavior coordination during fungal somatic cell fusion
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DOI：
10.1242/jcs.213462
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发表时间：
2018-05
影响因子：
4
通讯作者：
A. Serrano;J. Illgen;U. Brandt;Nils Thieme;A. Letz;Alexander Lichius;N. Read;A. Fleissner
中科院分区：
文献类型：
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作者：
A. Serrano;J. Illgen;U. Brandt;Nils Thieme;A. Letz;Alexander Lichius;N. Read;A. Fleissner
有丝分裂原活化蛋白激酶（MAPK）是真核细胞增殖、分化和适应的保守调节因子。它们的活动通常涉及它们的亚细胞定位的变化，表明这些时空动态在信号传输中的重要作用。说明这些动力学的一个引人注目的模型是粗糙脉孢菌中的体细胞融合。这种真菌的萌发孢子在信号发送和接收之间迅速交替，从而建立细胞-细胞对话，这涉及两种融合伴侣中MAPK MAK-2的交替膜募集。在这里，我们表明，MAK-2的动态易位是必不可少的协调行为的融合伙伴之前的物理接触。激酶的激活和功能与其亚细胞定位密切相关，表明MAPK动力学在建立调节反馈回路中的重要贡献，该调节反馈回路建立振荡信号模式。此外，我们提供的证据表明，MAK-2不仅有助于细胞间通讯，而且还介导细胞间融合。MAK-2的动力学显着不同，这两个过程之间，这表明MAPK在通信和融合之间的细胞程序的切换的作用。总结：MAPK的时空动态介导了融合粗糙脉孢菌细胞之间的相互作用，细胞间通过轮流发送和接收信号进行通讯。
ABSTRACT Mitogen-activated protein kinases (MAPKs) are conserved regulators of proliferation, differentiation and adaptation in eukaryotic cells. Their activity often involves changes in their subcellular localization, indicating an important role for these spatio-temporal dynamics in signal transmission. A striking model illustrating these dynamics is somatic cell fusion in Neurospora crassa. Germinating spores of this fungus rapidly alternate between signal sending and receiving, thereby establishing a cell-cell dialog, which involves the alternating membrane recruitment of the MAPK MAK-2 in both fusion partners. Here, we show that the dynamic translocation of MAK-2 is essential for coordinating the behavior of the fusion partners before physical contact. The activation and function of the kinase strongly correlate with its subcellular localization, indicating a crucial contribution of the MAPK dynamics in establishing regulatory feedback loops, which establish the oscillatory signaling mode. In addition, we provide evidence that MAK-2 not only contributes to cell-cell communication, but also mediates cell-cell fusion. The MAK-2 dynamics significantly differ between these two processes, suggesting a role for the MAPK in switching of the cellular program between communication and fusion. Summary: Spatio-temporal dynamics of MAPK mediate the interaction of fusing Neurospora crassa cells, which communicate by taking turns in signal sending and receiving.