The language of biological fluids
The language of biological fluids
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生物体液的语言
DOI：
10.1351/pac197850030205
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发表时间：
1978
影响因子：
1.8
通讯作者：
G. Spiteller
中科院分区：
文献类型：
--
作者：
G. Spiteller
摘要玻璃毛细管气相色谱与质谱仪联用是研究生物体液中类固醇和酸类物质的有效工具。气相色谱图中显示的许多化合物仍然未知。它们的结构测定可以用微量化学反应和质谱学相结合的方法来完成。几个例子证明了“侧写”的用处：情绪压力会导致脱氢表雄酮(−)排泄量增加10-30倍。在某些多毛症患者中，脱氢表雄酮排泄量的增加甚至更高。身体应激表现为柠檬酸排泄增加和氨基酸结合物减少。血浆中的主要类固醇是脱氢表雄酮。尿毒症患者的主要类固醇为3β，17β-二羟基-5-雄烯。
Abstract Glass capillary gas chromatography in combination with mass spectrometry is an efficient tool for the investigation of steroids and acids occurring in biological fluids. Many of the compounds indicated in the gas chromatograms are still unknown. Their structure determination can be done with a combination of microchemical reactions and mass spectrometry. A few examples demonstrate the usefulness of “profiling”: Emotional stress cause a 10 − 30 fold increase in the excretion of dehydroepiandrosterone (DHEA). An even higher increase in the DHEA excretion is observed in some cases of hirsutism. Physical stress is manifested by an increase in the excretion of citric acid and a decrease in conjugates of amino acids. The main steroid in blood plasma is DHEA. In patients suffering from uremia the main steroid was found to be 3β, 17β-dihydroxy-5-androstene.