A pteridine adsorbent for affinity chromatography
A pteridine adsorbent for affinity chromatography
复制标题
一种用于亲和层析的蝶啶吸附剂
DOI：
10.1016/0014-5793(74)81160-9
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发表时间：
1974
期刊：
影响因子：
3.5
通讯作者：
R. Cotton
中科院分区：
文献类型：
--
作者：
R. Cotton
与传统的酶纯化方法相比，亲和层析[L]为酶学者提供了相当大的优势。本文所描述的工作扩展了目前用于多种底物酶的亲和层析的辅因子的范围[2-71]。3.1)会与含有还原的蝶啶辅因子2-氨基-6，7-二甲基-4-羟基5，6，7，%四氢蝶啶的吸附剂结合非常强，并且可以用强碱性缓冲液或辅因子本身洗脱。在该吸附剂正下方使用Sephadex G-25柱可确保蛋白质从极端pH的洗脱缓冲液中快速分离。
Affinity chromatography [l] offers enzymologists considerable advantages over traditional methods of enzyme purification. The work described in this paper extends the range [2--71 of cofactors currently in use for affinity chromatography of enzymes with more than one substrate.The results to be described indicate that the enzyme phenylalanine hydroxylase (EC 1.14. 3.1) will bind very strongly to an adsorbent which contains the reduced pteridine cofactor, 2-amino 6, 7-dimethyl-4-hydroxy 5, 6, 7,% tetrahydropteridine and that it can be eluted with a strongly alkaline buffer or the cofactor itself. The use of a Sephadex G-25 column immediately below this adsorbent ensured rapid separation of the protein from the extreme pH of the elution buffer.