Incorporation rate of GM1 ganglioside into mouse brain myelin: effect of aging and modification by hormones and other compounds.
Incorporation rate of GM1 ganglioside into mouse brain myelin: effect of aging and modification by hormones and other compounds.
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GM1 神经节苷脂进入小鼠脑髓磷脂的掺入率：衰老和激素和其他化合物修饰的影响。
DOI：
10.1007/978-1-4684-1200-0_20
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发表时间：
1984
影响因子：
--
通讯作者：
K. Kon
中科院分区：
文献类型：
--
作者：
S. Ando;Y. Tanaka;Y. Ono;K. Kon
检查髓磷脂中 GM1 神经节苷脂的周转率揭示了代谢活动中与年龄相关的变化。三个不同年龄组的小鼠被给予氧化氘，并每隔两周从小脑中制备髓磷脂。通过高效液相色谱法从总髓磷脂神经节苷脂中分离出GM1。 GM1 的氘代糖部分通过化学电离质谱法测定，提供了显着的准分子离子。该方法可以分别测定内部和外部半乳糖以及其他成分中氘的掺入情况。 GM1 与髓磷脂的结合率明显随着年龄的增长而降低。新合成的唾液酸与 GM1 的结合量低于其他糖，可能表明唾液酸的再利用率约为 50%。通过监测用化学试剂处理的动物中 GM1 的掺入率来检查外源因素改变髓磷脂代谢的可能性。结果表明，甲状腺素增强了 GM1 与成人脑髓磷脂的结合，而丙硫氧嘧啶则减少了这种结合。其他化学物质，雌二醇、S-腺苷甲硫氨酸和 LM1 神经节苷脂，对髓磷脂周转仅产生轻微影响。
The turnover rate of GM1 ganglioside in myelin was examined reveal age-related alterations in the metabolic activity. Three different age groups of mice were given deuterium oxide, and myelin was prepared from cerebella at intervals of two weeks. GM1 was isolated from the total myelin gangliosides by high performance liquid chromatography. Deuterated sugar moieties of GM1 were determined by chemical ionization-mass spectrometry which provided prominent quasimolecular ions. This method made it possible to determine separately the incorporation of deuterium into internal and external galactoses as well as other components. The incorporation rate of GM1 into myelin was clearly shown to be decreased with advancing age. Lower incorporation of newly synthesized sialic acid into GM1 than that of other sugars may indicate reutilization of sialic acid at about 50%. The possibility of modification of the myelin metabolism by exogenous factors was examined by monitoring the incorporation rate of GM1 in animals treated with chemical agents. It was revealed that thyroxine enhanced the incorporation of GM1 into adult brain myelin, whereas propylthiouracil reduced the incorporation. Other chemicals, estradiol, S-adenosylmethionine and LM1 ganglioside, showed only minor effects on the myelin turnover.