B lymphocyte-specific, Cre-mediated mutagenesis in mice
B lymphocyte-specific, Cre-mediated mutagenesis in mice
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DOI：
10.1093/nar/25.6.1317
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发表时间：
1997-03-15
影响因子：
14.9
通讯作者：
Rajewsky, K
中科院分区：
文献类型：
--
作者：
Rickert, RC;Roes, J;Rajewsky, K
将源于P1噬菌体的Cre/loxP位点特异性重组系统应用于基因打靶技术，使得在小鼠中进行基因的条件性缺失成为可能。为了选择性地修饰B淋巴细胞中的基因，我们培育了在B细胞谱系受限的CD19基因转录调控下表达Cre的小鼠（命名为CD19 - Cre小鼠）。在一个涉及CD19 - Cre小鼠与携带loxP侧翼底物的小鼠杂交的模型系统中，我们发现骨髓来源的前B细胞中的缺失效率为75 - 80%，在脾脏B细胞中这一效率提高到90 - 95%。
Adaptation of the P1 phage-derived Cre/loxP site-specific recombination system to the gene targeting technique allows for the conditional deletion of genes in mice. To selectively modify genes in B lymphocytes, we have generated mice (designated CD19-Cre) which express cre under the transcriptional control of the B lineage-restricted CD19 gene. In a model system involving the cross of CD19-Cre mice with mice bearing a loxP-flanked substrate, we find a deletion efficiency of 75-80% in bone marrow-derived pre-B cells that increases to 90-95% in splenic B cells.