Long noncoding RNA colon cancer associated transcript-1 promotes the proliferation, migration and invasion of cervical cancer
Long noncoding RNA colon cancer associated transcript-1 promotes the proliferation, migration and invasion of cervical cancer
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DOI：
10.3892/mmr.2017.7302
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发表时间：
2017-10-01
影响因子：
3.4
通讯作者：
Xin, Hong
中科院分区：
文献类型：
--
作者：
Jia, Ligang;Zhang, Yuan;Xin, Hong
以往的研究表明，长链非编码RNA（lncRNA）在包括宫颈癌在内的多种肿瘤的发生、转移和侵袭中起着重要作用。本研究旨在探讨结肠癌相关转录本1（CCAT 1）在宫颈癌转移和侵袭中的作用及其可能机制。采用逆转录-定量聚合酶链反应（RT-PCR）检测宫颈癌组织中lncRNA CCAT 1 mRNA的表达，分析lncRNA CCAT 1与宫颈癌转移的关系。通过MTT和Transwell实验分析lncRNA CCAT 1表达对HeLa细胞活力、迁移和侵袭的影响。结果表明，lncRNA CCAT 1在宫颈癌组织中的表达高于癌旁正常组织。lncRNA CCAT 1的高表达与宫颈癌的大小呈正相关，与宫颈癌的生存率相关。通过沉默CCAT 1抑制细胞活力、迁移和侵袭能力。本研究结果提示lncRNA CCAT 1在宫颈癌中高表达，可能在促进宫颈癌的进展和转移中起重要作用。
Previous studies have revealed significant roles for long noncoding RNA (lncRNA) in the tumorigenesis, metastasis and invasion of various tumors, including cervical cancer. The present study aimed to investigate the potential roles of lncRNA colon cancer associated transcript 1 (CCAT1) in the metastasis and invasion of cervical cancer, and to reveal the potential underlying mechanism. The mRNA expression of lncRNA CCAT1 in cervical cancer tissue was measured using the reverse transcription-quantitative polymerase chain reaction, and the association between lncRNA CCAT1 and the metastasis of cervical cancer was analyzed. The effects of lncRNA CCAT1 expression on HeLa cell viability, and migration and invasion were also analyzed by MTT and Transwell assays. The results demonstrated that lncRNA CCAT1 was highly expressed in the cervical cancer tissue compared with the adjacent normal tissue. High expression of lncRNA CCAT1 was positively associated with tumor size, and there was correlation between high lncRNA CCAT1 expression and a poor survival rate of cervical cancer. The cell viability, and migratory and invasive abilities were suppressed by silencing CCAT1. The results of the present study indicate that lncRNA CCAT1 was highly expressed in cervical cancer, and may serve important roles in promoting the progression and metastasis of cervical cancer.