Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism
Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism
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DOI：
10.1091/mbc.01-07-0337
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发表时间：
2002-01-01
影响因子：
3.3
通讯作者：
Gauthier-Rouvière, C
中科院分区：
文献类型：
--
作者：
Mary, S;Charrasse, S;Gauthier-Rouvière, C
钙粘蛋白介导的细胞间粘附是一个动态过程，在胚胎发育、细胞迁移和分化过程中受到调节。不同的钙粘蛋白在特定组织中表达，与它们在细胞类型识别中的作用一致。在这项研究中，我们研究了成纤维细胞和成肌细胞中N-钙粘蛋白依赖性细胞间接触的形成。与E-钙粘蛋白相反，内源性和异位表达的N-钙粘蛋白在细胞内和质膜池之间穿梭。N-钙粘蛋白依赖性细胞-细胞接触的初始形成是由N-钙粘蛋白的细胞内库向质膜的募集引起的。N-钙粘蛋白也定位于高尔基体和分泌和内吞囊泡。我们证明，细胞内的N-钙粘蛋白池是紧密相关的微管（MT）网络和连接的形成需要MT。此外，定位的N-钙粘蛋白的皮质是依赖于一个完整的F-肌动蛋白细胞骨架。我们表明，N-钙粘蛋白的运输需要MT网络以及MT相关运动驱动蛋白的活性。总之，我们提出，N-钙粘蛋白分布是一个受调节的过程，促进细胞-细胞接触的形成，通过MT网络控制连接的生物发生和营业额。
Cadherin-mediated cell-cell adhesion is a dynamic process that is regulated during embryonic development, cell migration, and differentiation. Different cadherins are expressed in specific tissues consistent with their roles in cell type recognition. In this study, we examine the formation of N-cadherin-dependent cell-cell contacts in fibroblasts and myoblasts. In contrast to E-cadherin, both endogenous and ectopically expressed N-cadherin shuttles between an intracellular and a plasma membrane pool. Initial formation of N-cadherin-dependent cell-cell contacts results from the recruitment of the intracellular pool of N-cadherin to the plasma membrane. N-cadherin also localizes to the Golgi apparatus and both secretory and endocytotic vesicles. We demonstrate that the intracellular pool of N-cadherin is tightly associated with the microtubule (MT) network and that junction formation requires MTs. In addition, localization of N-cadherin to the cortex is dependent on an intact F-actin cytoskeleton. We show that N-cadherin transport requires the MT network as well as the activity of the MT-associated motor kinesin. In conclusion, we propose that N-cadherin distribution is a regulated process promoted by cell-cell contact formation, which controls the biogenesis and turnover of the junctions through the MT network.