Significance of the Bacteriophage Treatment Schedule in Reducing Salmonella Colonization of Poultry
Significance of the Bacteriophage Treatment Schedule in Reducing Salmonella Colonization of Poultry
复制标题
DOI：
10.1128/aem.01257-12
复制
发表时间：
2012-09-01
影响因子：
4.4
通讯作者：
Llagostera, Montserrat
中科院分区：
文献类型：
--
作者：
Bardina, Carlota;Spricigo, Denis A.;Llagostera, Montserrat
沙门氏菌仍然是世界范围内食源性疾病的主要原因，鸡是这种人畜共患病原体的主要宿主。在减少肉鸡沙门氏菌定植的许多方法中，噬菌体提供了几个优点。在这项研究中，从我们的收藏中获得的三种噬菌体（UAB_Phi20，UAB_Phi78和UAB_Phi87），表现出广泛的宿主范围对肠道沙门氏菌血清型肠道沙门氏菌和肠道沙门氏菌血清型鼠伤寒沙门氏菌进行了表征形态，基因组大小和限制性模式。由三种噬菌体组成的混合物对S.肠杆菌和S.比单独的三种噬菌体中的任何一种都要好。此外，混合物能够裂解肠道沙门氏菌血清型Virchow、Hadar和Infantis。噬菌体混合物在降低S.使用不同的治疗方案在两种动物模型中检测鼠伤寒沙门氏菌。在小鼠模型中，当在细菌感染的同时给予鸡尾酒时，并在感染后6、24和30 h再次给予鸡尾酒时，获得50%的存活率。同样，在白色来航鸡无特异性病原体（SPF）模型中，当在细菌感染前1天或刚感染后1天给予噬菌体混合物，然后在感染后不同的日子再次给予噬菌体混合物时，获得了定义为鸡盲肠中沙门氏菌浓度降低的最佳结果。我们的研究结果表明，频繁处理的鸡与噬菌体，特别是之前的肠道沙门氏菌定植，需要实现有效的细菌减少随着时间的推移。
Salmonella remains the major cause of food-borne diseases worldwide, with chickens known to be the main reservoir for this zoonotic pathogen. Among the many approaches to reducing Salmonella colonization of broilers, bacteriophage offers several advantages. In this study, three bacteriophages (UAB_Phi20, UAB_Phi78, and UAB_Phi87) obtained from our collection that exhibited a broad host range against Salmonella enterica serovar Enteritidis and Salmonella enterica serovar Typhimurium were characterized with respect to morphology, genome size, and restriction patterns. A cocktail composed of the three bacteriophages was more effective in promoting the lysis of S. Enteritidis and S. Typhimurium cultures than any of the three bacteriophages alone. In addition, the cocktail was able to lyse the Salmonella enterica serovars Virchow, Hadar, and Infantis. The effectiveness of the bacteriophage cocktail in reducing the concentration of S. Typhimurium was tested in two animal models using different treatment schedules. In the mouse model, 50% survival was obtained when the cocktail was administered simultaneously with bacterial infection and again at 6, 24, and 30 h postinfection. Likewise, in the White Leghorn chicken specific-pathogen-free (SPF) model, the best results, defined as a reduction of Salmonella concentration in the chicken cecum, were obtained when the bacteriophage cocktail was administered 1 day before or just after bacterial infection and then again on different days postinfection. Our results show that frequent treatment of the chickens with bacteriophage, and especially prior to colonization of the intestinal tract by Salmonella, is required to achieve effective bacterial reduction over time.