Isolation of Borna disease virus from human brain tissue
Isolation of Borna disease virus from human brain tissue
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DOI：
10.1128/jvi.74.10.4601-4611.2000
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发表时间：
2000-05-01
影响因子：
5.4
通讯作者：
Ikuta, K
中科院分区：
文献类型：
--
作者：
Nakamura, Y;Takahashi, H;Ikuta, K
血清学和分子流行病学研究表明，博尔纳病病毒（BDV）可感染人类，并可能与某些神经精神疾病有关。我们检查了从四名精神分裂症患者和两名健康对照尸检收集的脑组织中存在的BDV标记物在12个不同的大脑区域。在最近（2年）发病的BDV血清阳性精神分裂症患者（P2）的4个脑区中检测到BDV RNA和抗原。BDV标记表现出区域定位分布。新生蒙古沙鼠脑内接种的匀浆从BDV阳性脑区的P2，发现BDV RNA。用来自BDV阳性沙鼠的脑匀浆接种的人少突胶质细胞（OL）允许BDVHuP2br的增殖和分离，人脑来源的BDV病毒分离也可以通过用从BDV阳性人和沙鼠脑组织制备的核糖核蛋白复合物转染Vero细胞来实现。BDVHuP2br基因密切相关，但不同于以前报道的人类和动物来源的BDV序列。
Serological and molecular epidemiological studies indicate that Borna disease virus (BDV) can infect humans and is possibly associated with certain neuropsychiatric disorders. We examined brain tissue collected at autopsy from four schizophrenic patients and two healthy controls for the presence of BDV markers in 12 different brain regions. BDV RNA and antigen was detected in four brain regions of a BDV-seropositive schizophrenic patient (P2) with a very recent (2 years) onset of disease. BDV markers exhibited a regionally localized distribution. BDV RNA was found in newborn Mongolian gerbils intracranially inoculated with homogenates from BDV-positive brain regions of P2. Human oligodendroglia (OL) cells inoculated with brain homogenates from BDV-positive gerbils allowed propagation and isolation of BDVHuP2br, a human brain-derived BDV Virus isolation was also possible by transfection of Vero cells with ribonucleoprotein complexes prepared from BDV-positive human and gerbil brain tissues. BDVHuP2br was genetically closely related to but distinct from previously reported human- and animal-derived BDV sequences.