The Functional Assessment of LRRK2 in Caenorhabditis elegans Mechanosensory Neurons.
The Functional Assessment of LRRK2 in Caenorhabditis elegans Mechanosensory Neurons.
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DOI：
10.1007/978-1-0716-1495-2_17
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发表时间：
2021
影响因子：
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通讯作者：
Tomoki Kuwahara
中科院分区：
文献类型：
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作者：
Tomoki Kuwahara
秀丽隐杆线虫（C. habditiselegans，C. elegans）是系统分析体内感兴趣基因功能的强大模式生物。尤其是C。elegansnervous system由于其身体的光学透明性和包括神经连接在内的完善的解剖结构，适用于神经元基因的形态和功能分析。C.帕金森病相关基因LRRK 2（命名为lrk-1）的elegansortholog已被证明在调节神经元亚群的轴突形态中起作用。在这里，我描述了详细的方法来评估LRK-1/LRRK 2的功能，以及分析的遗传相互作用涉及lrk-1/LRRK 2通过执行活成像C。优雅的机械感受神经元。
The nematodeCaenorhabditis elegans(C. elegans) is a powerful model organism to systematically analyze the functions of genes of interest in vivo. Especially,C. elegansnervous system is suitable for morphological and functional analyses of neuronal genes due to its optical transparency of the body and the well-established anatomy including neural connections. TheC. elegansortholog of Parkinson’s disease-associated geneLRRK2, namedlrk-1, has been shown to play a role in the regulation of axonal morphology in a subset of neurons. Here I describe the detailed methodologies for the assessment of LRK-1/LRRK2 function as well as the analysis of genetic interaction involvinglrk-1/LRRK2by performing live imaging ofC. elegansmechanosenrory neurons.